Sulfatase-modifying factor: Difference between revisions
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<scene name='80/801257/Cv/9'>Glycosylated human sulfatase-modifying factor 1 complex with arylsulfatase peptide, Ca+2 ion and Cl- ion</scene>. The structure of SUMF1 complex with its substrate sulfatase terminal peptide CTPSR. <scene name='80/801257/Cv/10'>SUMF1 active site contains 2 cysteine residues and mutating either of them to serine results in an inactive enzyme</scene>. Cys341 was found to be <scene name='80/801257/Cv/11'>responsible for substrate binding and makes a Cys-Cys bond</scene> to the peptide cysteine residue. The peptide binds at the surface of SUMF1 in an extended conformation <scene name='80/801257/Cv/13'>making numerous interactions with the protein</scene><ref>PMID:16368756</ref>. | <scene name='80/801257/Cv/9'>Glycosylated human sulfatase-modifying factor 1 complex with arylsulfatase peptide, Ca+2 ion and Cl- ion</scene>. The structure of SUMF1 complex with its substrate sulfatase terminal peptide CTPSR. <scene name='80/801257/Cv/10'>SUMF1 active site contains 2 cysteine residues and mutating either of them to serine results in an inactive enzyme</scene>. Cys341 was found to be <scene name='80/801257/Cv/11'>responsible for substrate binding and makes a Cys-Cys bond</scene> to the peptide cysteine residue. The peptide binds at the surface of SUMF1 in an extended conformation <scene name='80/801257/Cv/13'>making numerous interactions with the protein</scene><ref>PMID:16368756</ref>. | ||
*<scene name='80/801257/Cv/14'>Cl coordination site</scene>. | *<scene name='80/801257/Cv/14'>Cl coordination site</scene>. Water molecules are shown as red spheres. | ||
*<scene name='80/801257/Cv/15'>1st Ca coordination site</scene>. | *<scene name='80/801257/Cv/15'>1st Ca coordination site</scene>. | ||
*<scene name='80/801257/Cv/16'>2nd Ca coordination site</scene>. | *<scene name='80/801257/Cv/16'>2nd Ca coordination site</scene>. | ||
