2vl6: Difference between revisions

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[[Image:2vl6.~~jpg~~|left|200px]]

[[Image:2vl6.png|left|200px]]

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~~'''~~STRUCTURAL ANALYSIS OF THE SULFOLOBUS SOLFATARICUS MCM PROTEIN N-TERMINAL DOMAIN~~'''~~

===STRUCTURAL ANALYSIS OF THE SULFOLOBUS SOLFATARICUS MCM PROTEIN N-TERMINAL DOMAIN===

~~==Overview==~~

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The ~~Mini-Chromosome Maintenance (MCM) proteins are candidates of replicative DNA helicase in eukarya and archaea. Here we report a 2.8 A crystal structure of~~ the ~~N-terminal domain (residues 1-268) of~~ the ~~Sulfolobus solfataricus MCM~~ (~~Sso MCM) protein~~. ~~The structure reveals single-hexameric ring-like architecture, at variance from the protein of Methanothermobacter thermoautotrophicus (Mth~~)~~. Moreover~~, the central channel in Sso MCM seems significantly narrower than the Mth counterpart, which appears to more favorably accommodate single-stranded DNA than double-stranded DNA, as supported by DNA-binding assays. ~~Structural analysis also highlights the essential role played by the zinc~~-~~binding domain in the interaction with nucleic acids and allows us to speculate that the Sso MCM N~~-~~ter domain may function as a molecular clamp to grasp the single-stranded DNA passing through the central channel. On this basis possible DNA unwinding mechanisms are discussed.~~

The line below this paragraph, {{ABSTRACT_PUBMED_18417534}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 18417534 is the PubMed ID number.

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==About this Structure==

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[[Category: Ssdna binding]]

[[Category: Zinc-finger]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Apr 30 13~~:24:14 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:39:12 2008''

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-[[Image:2vl6.jpg|left|200px]]
+{{Seed}}
+[[Image:2vl6.png|left|200px]]
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 {{STRUCTURE_2vl6|  PDB=2vl6  |  SCENE=  }}
-'''STRUCTURAL ANALYSIS OF THE SULFOLOBUS SOLFATARICUS MCM PROTEIN N-TERMINAL DOMAIN'''
+===STRUCTURAL ANALYSIS OF THE SULFOLOBUS SOLFATARICUS MCM PROTEIN N-TERMINAL DOMAIN===
-==Overview==
+<!--
-The Mini-Chromosome Maintenance (MCM) proteins are candidates of replicative DNA helicase in eukarya and archaea. Here we report a 2.8 A crystal structure of the N-terminal domain (residues 1-268) of the Sulfolobus solfataricus MCM (Sso MCM) protein. The structure reveals single-hexameric ring-like architecture, at variance from the protein of Methanothermobacter thermoautotrophicus (Mth). Moreover, the central channel in Sso MCM seems significantly narrower than the Mth counterpart, which appears to more favorably accommodate single-stranded DNA than double-stranded DNA, as supported by DNA-binding assays. Structural analysis also highlights the essential role played by the zinc-binding domain in the interaction with nucleic acids and allows us to speculate that the Sso MCM N-ter domain may function as a molecular clamp to grasp the single-stranded DNA passing through the central channel. On this basis possible DNA unwinding mechanisms are discussed.
+The line below this paragraph, {{ABSTRACT_PUBMED_18417534}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18417534 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18417534}}
 ==About this Structure==
@@ Line 37: / Line 41: @@
 [[Category: Ssdna binding]]
 [[Category: Zinc-finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:24:14 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:39:12 2008''