2zat: Difference between revisions

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[[Image:2zat.~~jpg~~|left|200px]]

[[Image:2zat.png|left|200px]]

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~~'''~~Crystal structure of a mammalian reductase~~'''~~

===Crystal structure of a mammalian reductase===

~~==Overview==~~

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~~Pig heart peroxisomal carbonyl reductase (PerCR) belongs to the short-chain dehydrogenase/reductase family~~, ~~and its sequence comprises a C-terminal SRL tripeptide~~, ~~which is a variant of~~ the ~~type 1 peroxisomal targeting signal~~ (~~PTS1) Ser-Lys-Leu. PerCR is imported into peroxisomes of HeLa cells when the cells are transfected with vectors expressing the enzyme~~. ~~However, PerCR does not show specific targeting when introduced into the cells with a protein transfection reagent~~. ~~To understand the structural basis for peroxisomal localization of PerCR~~, ~~we determined~~ the ~~crystal structure of PerCR~~. ~~Our data revealed that the C~~-terminal PTS1 of each subunit of PerCR was involved in intersubunit interactions and was buried in the interior of the tetrameric molecule. These findings indicate that the PTS1 receptor Pex5p in the cytosol recognizes the monomeric form of PerCR whose C-~~terminal PTS1 is exposed, and that this PerCR is targeted into the peroxisome, thereby forming a tetramer.~~

The line below this paragraph, {{ABSTRACT_PUBMED_18334214}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 18334214 is the PubMed ID number.

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==About this Structure==

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[[Category: Alpha/beta]]

[[Category: Oxidoreductase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Apr 30 13~~:30:41 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:46:12 2008''

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-[[Image:2zat.jpg|left|200px]]
+{{Seed}}
+[[Image:2zat.png|left|200px]]
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 {{STRUCTURE_2zat|  PDB=2zat  |  SCENE=  }}
-'''Crystal structure of a mammalian reductase'''
+===Crystal structure of a mammalian reductase===
-==Overview==
+<!--
-Pig heart peroxisomal carbonyl reductase (PerCR) belongs to the short-chain dehydrogenase/reductase family, and its sequence comprises a C-terminal SRL tripeptide, which is a variant of the type 1 peroxisomal targeting signal (PTS1) Ser-Lys-Leu. PerCR is imported into peroxisomes of HeLa cells when the cells are transfected with vectors expressing the enzyme. However, PerCR does not show specific targeting when introduced into the cells with a protein transfection reagent. To understand the structural basis for peroxisomal localization of PerCR, we determined the crystal structure of PerCR. Our data revealed that the C-terminal PTS1 of each subunit of PerCR was involved in intersubunit interactions and was buried in the interior of the tetrameric molecule. These findings indicate that the PTS1 receptor Pex5p in the cytosol recognizes the monomeric form of PerCR whose C-terminal PTS1 is exposed, and that this PerCR is targeted into the peroxisome, thereby forming a tetramer.
+The line below this paragraph, {{ABSTRACT_PUBMED_18334214}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18334214 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18334214}}
 ==About this Structure==
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 [[Category: Alpha/beta]]
 [[Category: Oxidoreductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:30:41 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:46:12 2008''