6l3m: Difference between revisions
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The | ==Crystal Structure of the acyltransferase domain from the third module of the ansamitocin polyketide synthase== | ||
<StructureSection load='6l3m' size='340' side='right'caption='[[6l3m]], [[Resolution|resolution]] 1.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6l3m]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L3M FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E5U:2-methoxypropanedioic+acid'>E5U</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3m OCA], [http://pdbe.org/6l3m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3m RCSB], [http://www.ebi.ac.uk/pdbsum/6l3m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3m ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A few acyltransferase (AT) domains of modular polyketide synthases (PKSs) recruit acyl carrier protein (ACP)-linked extender units with unusual C2 substituents to confer functionalities that are not available in coenzyme A (CoA)-linked ones. Here, an AT specific for methoxymalonyl (MOM)-ACP in the third module of the ansamitocin PKS was structurally and biochemically characterized. The AT uses a conserved tryptophan at the entrance of the substrate binding tunnel to discriminate between different carriers. A W275R mutation switches its carrier specificity from the ACP protein to the CoA molecule. The acyl-AT complex structures clearly show that the MOM-ACP accepted by the AT has the 2S instead of the opposite 2R stereochemistry that is predicted according to the biosynthetic derivation from a D-glycolytic intermediate. Together, these results reveal the structural basis of ATs recognizing ACP-linked extender units in polyketide biosynthesis. | |||
Structural and Biochemical insights to the Recruitment of Acyl Carrier Protein-linked Extender Units in Ansamitocin Biosynthesis.,Zhang F, Ji H, Ali I, Deng Z, Bai L, Zheng J Chembiochem. 2019 Nov 27. doi: 10.1002/cbic.201900628. PMID:31777147<ref>PMID:31777147</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6l3m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Zhang, F]] | |||
[[Category: Zheng, J]] | [[Category: Zheng, J]] | ||
[[Category: | [[Category: Acp-linked extender unit]] | ||
[[Category: Acyltransferase]] | |||
[[Category: Biosynthesis]] | |||
[[Category: Carrier specificity]] | |||
[[Category: Polyketide]] | |||
[[Category: Transferase]] | |||
Revision as of 07:42, 18 December 2019
Crystal Structure of the acyltransferase domain from the third module of the ansamitocin polyketide synthase
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