2uyz: Difference between revisions
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==Overview== | ==Overview== | ||
The ubiquitin-related modifier SUMO regulates a wide range of cellular, processes by post-translational modification with one, or a chain of SUMO, molecules. Sumoylation is achieved by the sequential action of several, enzymes in which the E2, Ubc9, transfers SUMO from the E1 to the target, mostly with the help of an E3 enzyme. In this process, Ubc9 not only forms, a thioester bond with SUMO, but also interacts with SUMO noncovalently., Here, we show that this noncovalent interaction promotes the formation of, short SUMO chains on targets such as Sp100 and HDAC4. We present a crystal, structure of the noncovalent Ubc9-SUMO1 complex, showing that SUMO is, located far from the E2 active site and resembles the noncovalent, interaction site for ubiquitin on UbcH5c and Mms2. Structural comparison, suggests a model for poly-sumoylation involving a mechanism analogous to, Mms2-Ubc13-mediated ubiquitin chain formation. | The ubiquitin-related modifier SUMO regulates a wide range of cellular, processes by post-translational modification with one, or a chain of SUMO, molecules. Sumoylation is achieved by the sequential action of several, enzymes in which the E2, Ubc9, transfers SUMO from the E1 to the target, mostly with the help of an E3 enzyme. In this process, Ubc9 not only forms, a thioester bond with SUMO, but also interacts with SUMO noncovalently., Here, we show that this noncovalent interaction promotes the formation of, short SUMO chains on targets such as Sp100 and HDAC4. We present a crystal, structure of the noncovalent Ubc9-SUMO1 complex, showing that SUMO is, located far from the E2 active site and resembles the noncovalent, interaction site for ubiquitin on UbcH5c and Mms2. Structural comparison, suggests a model for poly-sumoylation involving a mechanism analogous to, Mms2-Ubc13-mediated ubiquitin chain formation. | ||
==Disease== | |||
Known diseases associated with this structure: Orofacial cleft 10 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601912 601912]] | |||
==About this Structure== | ==About this Structure== | ||
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[[Category: ubl conjugation pathway]] | [[Category: ubl conjugation pathway]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:40:26 2007'' | ||
