5ah1: Difference between revisions

Revision as of 04:59, 25 May 2023

Structure of EstA from Clostridium botulinum

Structural highlights

5ah1 is a 1 chain structure with sequence from Clostridium botulinum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	K, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A5I055_CLOBH

Publication Abstract from PubMed

Two novel esterases from the anaerobe Clostridium botulinum ATCC 3502 (Cbotu_EstA and Cbotu_EstB) were expressed in Escherichia coli BL21-Gold(DE3) and were found to hydrolyze the polyester poly(butylene adipate-co-butylene terephthalate) (PBAT). The active site residues (triad Ser, Asp, His) are present in both enzymes at the same location only with some amino acid variations near the active site at the surrounding of aspartate. Yet, Cbotu_EstA showed higher kcat values on para-nitrophenyl butyrate and para-nitrophenyl acetate and was considerably more active (sixfold) on PBAT. The entrance to the active site of the modeled Cbotu_EstB appears more narrowed compared to the crystal structure of Cbotu_EstA and the N-terminus is shorter which could explain its lower activity on PBAT. The Cbotu_EstA crystal structure consists of two regions that may act as movable cap domains and a zinc metal binding site. Biotechnol. Bioeng. 2016;113: 1024-1034. (c) 2015 Wiley Periodicals, Inc.

Hydrolysis of synthetic polyesters by Clostridium botulinum esterases.,Perz V, Baumschlager A, Bleymaier K, Zitzenbacher S, Hromic A, Steinkellner G, Pairitsch A, Lyskowski A, Gruber K, Sinkel C, Kuper U, Ribitsch D, Guebitz GM Biotechnol Bioeng. 2016 May;113(5):1024-34. doi: 10.1002/bit.25874. Epub 2015 Nov, 20. PMID:26524601^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Perz V, Baumschlager A, Bleymaier K, Zitzenbacher S, Hromic A, Steinkellner G, Pairitsch A, Lyskowski A, Gruber K, Sinkel C, Kuper U, Ribitsch D, Guebitz GM. Hydrolysis of synthetic polyesters by Clostridium botulinum esterases. Biotechnol Bioeng. 2016 May;113(5):1024-34. doi: 10.1002/bit.25874. Epub 2015 Nov, 20. PMID:26524601 doi:https://dx.doi.org/10.1002/bit.25874

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Perz V, Baumschlager A, Bleymaier K, Zitzenbacher S, Hromic A, Steinkellner G, Pairitsch A, Lyskowski A, Gruber K, Sinkel C, Kuper U, Ribitsch D, Guebitz GM. Hydrolysis of synthetic polyesters by Clostridium botulinum esterases. Biotechnol Bioeng. 2016 May;113(5):1024-34. doi: 10.1002/bit.25874. Epub 2015 Nov, 20. PMID:26524601 doi:https://dx.doi.org/10.1002/bit.25874

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='5ah1' size='340' side='right'caption='[[5ah1]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ah1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AH1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ah1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AH1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ah1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ah1 OCA], [https://pdbe.org/5ah1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ah1 RCSB], [https://www.ebi.ac.uk/pdbsum/5ah1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ah1 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ah1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ah1 OCA], [http://pdbe.org/5ah1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ah1 RCSB], [http://www.ebi.ac.uk/pdbsum/5ah1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ah1 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A5I055_CLOBH A5I055_CLOBH]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus botulinus van ermengem 1896]]
+[[Category: Clostridium botulinum]]
 [[Category: Large Structures]]
-[[Category: Triacylglycerol lipase]]
+[[Category: Baumschlager A]]
-[[Category: Baumschlager, A]]
+[[Category: Bleymaier K]]
-[[Category: Bleymaier, K]]
+[[Category: Gruber K]]
-[[Category: Gruber, K]]
+[[Category: Guebitz GM]]
-[[Category: Guebitz, G M]]
+[[Category: Hromic A]]
-[[Category: Hromic, A]]
+[[Category: Kueper U]]
-[[Category: Kueper, U]]
+[[Category: Lyskowski A]]
-[[Category: Lyskowski, A]]
+[[Category: Pairitsch A]]
-[[Category: Pairitsch, A]]
+[[Category: Perz V]]
-[[Category: Perz, V]]
+[[Category: Ribitsch D]]
-[[Category: Ribitsch, D]]
+[[Category: Sinkel C]]
-[[Category: Sinkel, C]]
+[[Category: Steinkellner G]]
-[[Category: Steinkellner, G]]
+[[Category: Zitzenbacher S]]
-[[Category: Zitzenbacher, S]]
-[[Category: Alpha/beta- hydrolase]]
-[[Category: Hydrolase]]
-[[Category: Polyesterase]]
-[[Category: Polymer hydrolysis]]
-[[Category: Zinc binding]]

5ah1: Difference between revisions

Revision as of 04:59, 25 May 2023

Structure of EstA from Clostridium botulinum

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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