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| [[Image:12e8.gif|left|200px]] | | {{Seed}} |
| | [[Image:12e8.png|left|200px]] |
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| {{STRUCTURE_12e8| PDB=12e8 | SCENE= }} | | {{STRUCTURE_12e8| PDB=12e8 | SCENE= }} |
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| '''2E8 FAB FRAGMENT'''
| | ===2E8 FAB FRAGMENT=== |
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| ==Overview==
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| The crystal structure of the Fab fragment of 2E8, the monoclonal IgG1,kappa antibody specific for the low-density lipoprotein (LDL) receptor-binding region of apolipoprotein E (apoE), has been solved by molecular replacement and refined at 1.9 A resolution (PDB entry 12E8). Two 2E8 Fab molecules in the asymmetric unit are related by noncrystallographic symmetry and are hydrogen bonded through a beta-sheet-like intermolecular contact between the heavy-chain complementarity-determining regions 3 (CDRH3) of each molecule. The structure has been refined to an R value of 0.22 (Rfree = 0.27). The initially ill-defined heavy-chain constant domain (CH1) of 2E8 has been retraced with the aid of automatic refinement, confirming the beta-sheet tracing independently of any starting models. As a resolution better than 2 A is not common for Fab fragments, this model represents a well defined Fab structure and should prove useful in MR solution of other Fab fragments. Furthermore, in the absence of an LDL-receptor structure, the homology of the 2E8 CDRH2 to the ligand-binding domain of the LDL receptor has been exploited to model the apoE-LDL-receptor interaction. | | The line below this paragraph, {{ABSTRACT_PUBMED_10089402}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10089402 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10089402}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Trakhanov, S.]] | | [[Category: Trakhanov, S.]] |
| [[Category: Immunoglobulin]] | | [[Category: Immunoglobulin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:28:33 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:26:54 2008'' |
