6l2f: Difference between revisions

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'''Unreleased structure'''


The entry 6l2f is ON HOLD  until Paper Publication
==Crystal structure of a cupin protein (tm1459, H54AH58A mutant) in copper (Cu) substituted form==
<StructureSection load='6l2f' size='340' side='right'caption='[[6l2f]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6l2f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L2F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L2F FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l2f OCA], [http://pdbe.org/6l2f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l2f RCSB], [http://www.ebi.ac.uk/pdbsum/6l2f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l2f ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cupin superfamily proteins (TM1459) work as a macromolecular ligand framework with a double-stranded beta-barrel structure ligating to a Cu ion through histidine side chains. Variegating the first coordination sphere of TM1459 revealed that H52A and H54A/H58A mutants effectively catalyzed the diastereo- and enantioselective Michael addition reaction of nitroalkanes to an alpha,beta-unsaturated ketone. Moreover, calculated substrate docking signified C106N and F104W single-point mutations, which inverted the diastereoselectivity of H52A and further improved the stereoselectivity of H54A/H58A, respectively.


Authors: Fujieda, N., Ichihashi, H., Nishikawa, Y., Kurisu, G., Itoh, S.
Cupin Variants as a Macromolecular Ligand Library for Stereoselective Michael Addition of Nitroalkanes.,Fujieda N, Ichihashi H, Yuasa M, Nishikawa Y, Kurisu G, Itoh S Angew Chem Int Ed Engl. 2020 Feb 19. doi: 10.1002/anie.202000129. PMID:32073197<ref>PMID:32073197</ref>


Description: Crystal structure of a cupin protein (tm1459, H54AH58A mutant) in copper (Cu) substituted form
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 6l2f" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Fujieda, N]]
[[Category: Ichihashi, H]]
[[Category: Ichihashi, H]]
[[Category: Itoh, S]]
[[Category: Itoh, S]]
[[Category: Kurisu, G]]
[[Category: Nishikawa, Y]]
[[Category: Nishikawa, Y]]
[[Category: Kurisu, G]]
[[Category: Artificial metalloenzyme]]
[[Category: Fujieda, N]]
[[Category: Copper enzyme]]
[[Category: Cupin]]
[[Category: Metal binding protein]]
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