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| [[Image:194l.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_194l| PDB=194l | SCENE= }} | | {{STRUCTURE_194l| PDB=194l | SCENE= }} |
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| '''THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME'''
| | ===THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME=== |
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| ==Overview==
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| Crystals of tetragonal hen egg-white lysozyme were grown using Advanced Protein Crystallization Facility (APCF) apparatus under a microgravity environment (SpaceHab-01 mission) and ground control conditions. Crystals were grown from NaCl as a crystallizing agent at pH 4.3. The X-ray diffraction patterns of the best diffracting ground- and space-grown crystals were recorded using synchrotron radiation and an image plate on the W32 beamline at LURE. Both ground- and space-grown crystals showed nearly equivalent maximum resolution of 1.3-1.4 A. Refinements were carried out with the program X-PLOR with final R values of 18.45 and 18.27% for structures from ground- and space- grown crystals, respectively. The two structures are nearly identical with the root-mean-square difference on all protein atoms being 0.13 A. Some residues of the two refined structures show multiple alternative conformations. Two ions were localized into the electron-density maps of the two structures: one chloride ion at the interface between two symmetry-related molecules and one sodium ion stabilizing the loop Ser60-Leu75. The sodium ion is surrounded by six ligands which form a bipyramid around it at distances of 2.2-2.6 A.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15299672}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15299672 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15299672}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Ries-Kautt, M.]] | | [[Category: Ries-Kautt, M.]] |
| [[Category: Vaney, M C.]] | | [[Category: Vaney, M C.]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:35:27 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:40:06 2008'' |
