6v3z: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6v3z is ~~ON HOLD~~

==Structure of Salmonella enteritidis Sen1395==

== Structural highlights ==

<table><tr><td colspan='2'>[[6v3z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V3Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6V3Z FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6v3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v3z OCA], [http://pdbe.org/6v3z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v3z RCSB], [http://www.ebi.ac.uk/pdbsum/6v3z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v3z ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Typhoid toxin is a virulence factor for the bacterial pathogen Salmonella Typhi, which causes typhoid fever in humans. After its synthesis by intracellular bacteria, typhoid toxin is secreted into the lumen of the Salmonella-containing vacuole by a secretion mechanism strictly dependent on TtsA, a specific muramidase that facilitates toxin transport through the peptidoglycan layer. Here we show that substrate recognition by TtsA depends on a discrete domain within its carboxy terminus, which targets the enzyme to the bacterial poles to recognize YcbB-edited peptidoglycan. Comparison of the atomic structures of TtsA bound to its substrate and that of a close homolog with different specificity identified specific determinants involved in substrate recognition. Combined with structure-guided mutagenesis and in vitro and in vivo crosslinking experiments, this study provides an unprecedented view of the mechanisms by which a muramidase recognizes its peptidoglycan substrate to facilitate protein secretion.

~~Authors: Galan~~, ~~J.E.~~, Lara-Tejero, M.

Mechanisms of substrate recognition by a typhoid toxin secretion-associated muramidase.,Geiger T, Lara-Tejero M, Xiong Y, Galan JE Elife. 2020 Jan 20;9. pii: 53473. doi: 10.7554/eLife.53473. PMID:31958059<ref>PMID:31958059</ref>

~~Description~~: ~~Structure of Salmonella enteritidis Sen1395~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Galan, J E]]

[[Category: Lara-Tejero, M]]

[[Category: ~~Galan, J.E~~]]

[[Category: Muramidase lysozyme-like peptidoglycan-binding]]

[[Category: Protein transport]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6v3z is ON HOLD
+==Structure of Salmonella enteritidis Sen1395==
+<StructureSection load='6v3z' size='340' side='right'caption='[[6v3z]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6v3z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V3Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6V3Z FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6v3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v3z OCA], [http://pdbe.org/6v3z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v3z RCSB], [http://www.ebi.ac.uk/pdbsum/6v3z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v3z ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Typhoid toxin is a virulence factor for the bacterial pathogen Salmonella Typhi, which causes typhoid fever in humans. After its synthesis by intracellular bacteria, typhoid toxin is secreted into the lumen of the Salmonella-containing vacuole by a secretion mechanism strictly dependent on TtsA, a specific muramidase that facilitates toxin transport through the peptidoglycan layer. Here we show that substrate recognition by TtsA depends on a discrete domain within its carboxy terminus, which targets the enzyme to the bacterial poles to recognize YcbB-edited peptidoglycan. Comparison of the atomic structures of TtsA bound to its substrate and that of a close homolog with different specificity identified specific determinants involved in substrate recognition. Combined with structure-guided mutagenesis and in vitro and in vivo crosslinking experiments, this study provides an unprecedented view of the mechanisms by which a muramidase recognizes its peptidoglycan substrate to facilitate protein secretion.
-Authors: Galan, J.E., Lara-Tejero, M.
+Mechanisms of substrate recognition by a typhoid toxin secretion-associated muramidase.,Geiger T, Lara-Tejero M, Xiong Y, Galan JE Elife. 2020 Jan 20;9. pii: 53473. doi: 10.7554/eLife.53473. PMID:31958059<ref>PMID:31958059</ref>
-Description: Structure of Salmonella enteritidis Sen1395
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6v3z" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Galan, J E]]
 [[Category: Lara-Tejero, M]]
-[[Category: Galan, J.E]]
+[[Category: Muramidase lysozyme-like peptidoglycan-binding]]
+[[Category: Protein transport]]