6jlc

Structure determination of CAMP factor of Mobiluncus curtisii and insight into structural dynamics

spinqualitylabelsall models 6jlc, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6jlc is a 1 chain structure with sequence from Falcivibrio vaginalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT Gene: HMPREF0573_10472 (Falcivibrio vaginalis) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Bacterial vaginosis (BV) is a common type of vaginal inflammation caused by a proliferation of pathogenic bacteria, among which Mobiluncus curtisii. In our previous studies on M. curtisii genome, we identified the presence of a genomic fragment encoding a 25 kDa pore-forming toxin, the CAMP factor, which is known to be involved in the synergistic lysis of erythrocytes namely CAMP reaction. However, whether this hypothetical gene product has hemolytic activity is unknown. Moreover, its relative structure and function are not yet solved. Here we found that the M. curtisii CAMP factor is a monomer at pH 4.4 and oligomer at pH >4.6. Hemolysis assays showed that M. curtisii CAMP factor could lyse sheep red blood cells efficiently in pH 5.4-7.4. Negative staining electron microscope analysis of the CAMP factor revealed ring-like structures at pH above 4.6. Additionally, the crystal structure of M. curtisii CAMP factor, determineded at 1.85 A resolution, reveals a 5 + 3 helix motif. Further functional analysis suggested that the structural rearrangement of the N-terminal domain might be required for protein function. In conclusion, this structure-function relationship study of CAMP factor provides a new perspective of the M. curtisii role in BV development. Structure determination of CAMP factor of Mobiluncus curtisii and insights into structural dynamics.,Zeng W, Ma H, Fan W, Yang Y, Zhang C, Arnaud Kombe Kombe J, Fan X, Zhang Y, Dong Z, Shen Z, Zhou Y, Yang M, Jin T Int J Biol Macromol. 2019 Nov 15. pii: S0141-8130(19)37284-8. doi:, 10.1016/j.ijbiomac.2019.10.107. PMID:31739050 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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