5u92

Crystal Structure of arginine kinase from the spider Polybetes pythagoricus in complex with arginine

spinqualitylabelsall models 5u92, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5u92 is a 1 chain structure with sequence from Polybetes pythagoricus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: ARG, CSO, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function KARG_POLPT Publication Abstract from PubMed Energy buffering systems are key for homeostasis during variations in energy supply. Spiders are the most important predators for insects and therefore key in terrestrial ecosystems. From biomedical interest, spiders are important for their venoms and as a source of potent allergens, such as arginine kinase (AK, EC 2.7.3.3). AK is an enzyme crucial for energy metabolism, keeping the pool of phosphagens in invertebrates, and also an allergen for humans. In this work, we studied AK from the Argentininan spider Polybetes pythagoricus (PpAK), from its complementary DNA to the crystal structure. The PpAK cDNA from muscle was cloned, and it is comprised of 1068 nucleotides that encode a 384-amino acids protein, similar to other invertebrate AKs. The apparent Michaelis-Menten kinetic constant (Km ) was 1.7 mM with a kcat of 75 s-1. Two crystal structures are presented, the apoPvAK and PpAK bound to arginine, both in the open conformation with the active site lid (residues 310-320) completely disordered. The guanidino group binding site in the apo structure appears to be organized to accept the arginine substrate. Finally, these results contribute to knowledge of mechanistic details of the function of arginine kinase. Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus.,Laino A, Lopez-Zavala AA, Garcia-Orozco KD, Carrasco-Miranda JS, Santana M, Stojanoff V, Sotelo-Mundo RR, Garcia CF PeerJ. 2017 Sep 11;5:e3787. doi: 10.7717/peerj.3787. eCollection 2017. PMID:28924503 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Arginine kinase 3D structures

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