1a1u: Difference between revisions

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[[Image:1a1u.gif|left|200px]]
{{Seed}}
[[Image:1a1u.png|left|200px]]


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{{STRUCTURE_1a1u|  PDB=1a1u  |  SCENE=  }}  
{{STRUCTURE_1a1u|  PDB=1a1u  |  SCENE=  }}  


'''SOLUTION STRUCTURE DETERMINATION OF A P53 MUTANT DIMERIZATION DOMAIN, NMR, MINIMIZED AVERAGE STRUCTURE'''
===SOLUTION STRUCTURE DETERMINATION OF A P53 MUTANT DIMERIZATION DOMAIN, NMR, MINIMIZED AVERAGE STRUCTURE===




==Overview==
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The p53 tumor suppressor oligomerization domain, a dimer of two primary dimers, is an independently folding domain whose subunits consist of a beta-strand, a tight turn and an alpha-helix. To evaluate the effect of hydrophobic side-chains on three-dimensional structure, we substituted residues Phe341 and Leu344 in the alpha-helix with other hydrophobic amino acids. Substitutions that resulted in residue 341 having a smaller side-chain than residue 344 switched the stoichiometry of the domain from tetrameric to dimeric. The three-dimensional structure of one such dimer was determined by multidimensional NMR spectroscopy. When compared with the primary dimer of the wild-type p53 oligomerization domain, the mutant dimer showed a switch in alpha-helical packing from anti-parallel to parallel and rotation of the alpha-helices relative to the beta-strands. Hydrophobic side-chain size is therefore an important determinant of a protein fold.
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{{ABSTRACT_PUBMED_9321402}}


==About this Structure==
==About this Structure==
1A1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A1U OCA].  
1A1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A1U OCA].  


==Reference==
==Reference==
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[[Category: Oligomerization domain]]
[[Category: Oligomerization domain]]
[[Category: P53]]
[[Category: P53]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:40:55 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:48:34 2008''
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