Sandbox Reserved 1561: Difference between revisions

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<scene name='82/823085/Bap1_hydrophobic_polar_view/1'>Bap1 Hydrophobic Polar View</scene> Bap1 is an insoluble protein at full length and requires a deletion of a hydrophobic 57-amino acid chain, which forms the beta-prism domain within blade-six of the eight-bladed beta-propeller.

<scene name='82/823085/57aa_insertion/1'>57aa Insertion</scene>

<scene name='82/823085/57aa_insertion/1'>57aa Insertion</scene> is located in the beta-prism of Bap1 between residues 414-473. Bap1 eight-bladed beta-propeller is insoluble until the beta-prism is attached in the loop of blade six of the beta-propeller. Once the insertion and beta-prism are bound to the beta-propeller Bap1 improves in soluble expression.

<scene name='82/823085/Ligand_view/1'>Ligand View</scene> The ligands that make up Bap1 are FLC, GOL, NA and CA. Citrate (FLC) binds carbohydrates and promotes structural qualities. Glycerol (GOL) promotes adhesion. Sodium (NA) has one molecule per blade on the beta-propeller. Finally, calcium (CA) provides structure for the eight-bladed crystal structure.

Sandbox Reserved 1561: Difference between revisions

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@@ Line 14: / Line 14: @@
 <scene name='82/823085/Bap1_hydrophobic_polar_view/1'>Bap1 Hydrophobic Polar View</scene> Bap1 is an insoluble protein at full length and requires a deletion of a hydrophobic 57-amino acid chain, which forms the beta-prism domain within blade-six of the eight-bladed beta-propeller.
-<scene name='82/823085/57aa_insertion/1'>57aa Insertion</scene>
+<scene name='82/823085/57aa_insertion/1'>57aa Insertion</scene> is located in the beta-prism of Bap1 between residues 414-473. Bap1 eight-bladed beta-propeller is insoluble until the beta-prism is attached in the loop of blade six of the beta-propeller. Once the insertion and beta-prism are bound to the beta-propeller Bap1 improves in soluble expression.
 <scene name='82/823085/Ligand_view/1'>Ligand View</scene> The ligands that make up Bap1 are FLC, GOL, NA and CA. Citrate (FLC) binds carbohydrates and promotes structural qualities. Glycerol (GOL) promotes adhesion. Sodium (NA) has one molecule per blade on the beta-propeller. Finally, calcium (CA) provides structure for the eight-bladed crystal structure.