Sandbox Reserved 1561: Difference between revisions
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<scene name='82/823085/57aa_insertion/1'>57aa Insertion</scene> is located in the beta-prism of Bap1 between residues 414-473. Bap1 eight-bladed beta-propeller is insoluble until the beta-prism is attached in the loop of blade six of the beta-propeller. Once the insertion and beta-prism are bound to the beta-propeller Bap1 improves in soluble expression. <ref>Kaus, Katherine, et al. The 1.9 Å Crystal Structure of the Extracellular Matrix Protein Bap1 from Vibrio Cholerae Provides Insights into Bacterial Biofilm Adhesion. The American Society for Biochemistry and Molecular Biology, 2019</ref> | <scene name='82/823085/57aa_insertion/1'>57aa Insertion</scene> is located in the beta-prism of Bap1 between residues 414-473. Bap1 eight-bladed beta-propeller is insoluble until the beta-prism is attached in the loop of blade six of the beta-propeller. Once the insertion and beta-prism are bound to the beta-propeller Bap1 improves in soluble expression. <ref>Kaus, Katherine, et al. The 1.9 Å Crystal Structure of the Extracellular Matrix Protein Bap1 from Vibrio Cholerae Provides Insights into Bacterial Biofilm Adhesion. The American Society for Biochemistry and Molecular Biology, 2019</ref> | ||
<scene name='82/823085/Ligand_view/1'>Ligand View</scene> The ligands that make up Bap1 are FLC, GOL, NA and CA. Citrate (FLC) binds carbohydrates and promotes structural qualities. Glycerol (GOL) promotes adhesion. Sodium (NA) has one molecule per blade on the beta-propeller. Finally, calcium (CA) provides structure for the eight-bladed crystal structure. | <scene name='82/823085/Ligand_view/1'>Ligand View</scene> The ligands that make up Bap1 are FLC, GOL, NA and CA. Citrate (FLC) binds carbohydrates and promotes structural qualities. Glycerol (GOL) promotes adhesion. Sodium (NA) has one molecule per blade on the beta-propeller. Finally, calcium (CA) provides structure for the eight-bladed crystal structure. <ref>Kaus, Katherine, et al. The 1.9 Å Crystal Structure of the Extracellular Matrix Protein Bap1 from Vibrio Cholerae Provides Insights into Bacterial Biofilm Adhesion. The American Society for Biochemistry and Molecular Biology, 2019.</ref> | ||
<scene name='82/823085/Eight-bladed_beta-propeller/2'>Eight-Bladed Beta-Propeller</scene> The eight-bladed beta-propeller has a four-stranded antiparallel beta-sheet making up each blade. | <scene name='82/823085/Eight-bladed_beta-propeller/2'>Eight-Bladed Beta-Propeller</scene> The eight-bladed beta-propeller has a four-stranded antiparallel beta-sheet making up each blade. | ||
