1a3s: Difference between revisions

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[[Image:1a3s.jpg|left|200px]]
{{Seed}}
[[Image:1a3s.png|left|200px]]


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{{STRUCTURE_1a3s|  PDB=1a3s  |  SCENE=  }}  
{{STRUCTURE_1a3s|  PDB=1a3s  |  SCENE=  }}  


'''HUMAN UBC9'''
===HUMAN UBC9===




==Overview==
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The three-dimensional structure of ubiquitin-conjugating enzyme 9 (Ubc9) has been obtained to a resolution of 2.8 A by molecular replacement followed by a combination of automated refinement and graphical intervention. Diffraction data were recorded on a single crystal in space group P43 with cell dimensions a = b = 73.9, c = 42. 9 A. The final model has an R factor of 21.3% for all data to 2.8 A. Only the N-terminal methionine, a two-residue N-terminal extension and a four-residue loop are not located by the final electron-density map. Ubc9 is now known to be the first sumo, a new ubiquitin-like protein, conjugating enzyme and does not conjugate ubiquitin. The structure of Ubc9 shows important differences compared with the structures of known ubiquitin-conjugating enzymes. At the N-terminal helix, the structural and sequence alignments are out of register by one amino acid giving Ubc9 a different recognition surface compared to ubiquitin-conjugating enzymes. This is coupled to a profound change in the electrostatic surface of the molecular face remote from the catalytic site. These differences may be important in recognition of other proteins in the Sumo conjugation pathway. The catalytic cysteine in Ubc9 has a positively charged lip and a negatively charged ridge nearby. Both these features seem confined to sumo-conjugating enzymes, and a sequence alignment of sumo and ubiquitin suggests how these might play a role in sumo/ubiquitin discrimination.
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{{ABSTRACT_PUBMED_9757105}}


==About this Structure==
==About this Structure==
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[[Category: Sumo conjugating enzyme]]
[[Category: Sumo conjugating enzyme]]
[[Category: Ubiquitin conjugating enzyme]]
[[Category: Ubiquitin conjugating enzyme]]
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Revision as of 12:57, 30 June 2008

File:1a3s.png

Template:STRUCTURE 1a3s

HUMAN UBC9

Template:ABSTRACT PUBMED 9757105

About this Structure

1A3S is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of ubiquitin-conjugating enzyme 9 displays significant differences with other ubiquitin-conjugating enzymes which may reflect its specificity for sumo rather than ubiquitin., Giraud MF, Desterro JM, Naismith JH, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):891-8. PMID:9757105

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