6sgc

Rabbit 80S ribosome stalled on a poly(A) tail

Loading... Status: waiting for Mol* initialization Structural highlights 6sgc is a 86 chain structure with sequence from European rabbit and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, SPD, ZN NonStd Res: 12A, 1MA, 2MU, 5MC, 70U, 7MG, H2U, PSU Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [U3KPD5_RABIT] Binds to the 23S rRNA.[RuleBase:RU000576] [G1SS70_RABIT] May play a role during erythropoiesis through regulation of transcription factor DDIT3.[HAMAP-Rule:MF_03122] Publication Abstract from PubMed Faulty or damaged messenger RNAs are detected by the cell when translating ribosomes stall during elongation and trigger pathways of mRNA decay, nascent protein degradation and ribosome recycling. The most common mRNA defect in eukaryotes is probably inappropriate polyadenylation at near-cognate sites within the coding region. How ribosomes stall selectively when they encounter poly(A) is unclear. Here, we use biochemical and structural approaches in mammalian systems to show that poly-lysine, encoded by poly(A), favors a peptidyl-transfer RNA conformation suboptimal for peptide bond formation. This conformation partially slows elongation, permitting poly(A) mRNA in the ribosome's decoding center to adopt a ribosomal RNA-stabilized single-stranded helix. The reconfigured decoding center clashes with incoming aminoacyl-tRNA, thereby precluding elongation. Thus, coincidence detection of poly-lysine in the exit tunnel and poly(A) in the decoding center allows ribosomes to detect aberrant mRNAs selectively, stall elongation and trigger downstream quality control pathways essential for cellular homeostasis. Mechanism of ribosome stalling during translation of a poly(A) tail.,Chandrasekaran V, Juszkiewicz S, Choi J, Puglisi JD, Brown A, Shao S, Ramakrishnan V, Hegde RS Nat Struct Mol Biol. 2019 Nov 25. pii: 10.1038/s41594-019-0331-x. doi:, 10.1038/s41594-019-0331-x. PMID:31768042 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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