6tzk: Difference between revisions

Latest revision as of 10:32, 15 February 2023

Bacterial cellulose synthase outermembrane channel BcsC with terminal TPR repeat

Structural highlights

6tzk is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	3PE, C8E, CA, HEZ, LMT, PE5
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BCSC_ECOLI Required for maximal bacterial cellulose synthesis.

Publication Abstract from PubMed

Extracellular bacterial cellulose contributes to biofilm stability and to the integrity of the bacterial cell envelope. In Gram-negative bacteria, cellulose is synthesized and secreted by a multi-component cellulose synthase complex. The BcsA subunit synthesizes cellulose and also transports the polymer across the inner membrane. Translocation across the outer membrane occurs through the BcsC porin, which extends into the periplasm via 19 tetra-tricopeptide repeats (TPR). We present the crystal structure of a truncated BcsC, encompassing the last TPR repeat and the complete outer membrane channel domain, revealing a 16-stranded, beta barrel pore architecture. The pore is blocked by an extracellular gating loop, while the extended C terminus inserts deeply into the channel and positions a conserved Trp residue near its extracellular exit. The channel is lined with hydrophilic and aromatic residues suggesting a mechanism for facilitated cellulose diffusion based on aromatic stacking and hydrogen bonding.

Architecture of the Cellulose Synthase Outer Membrane Channel and Its Association with the Periplasmic TPR Domain.,Acheson JF, Derewenda ZS, Zimmer J Structure. 2019 Oct 1. pii: S0969-2126(19)30314-4. doi:, 10.1016/j.str.2019.09.008. PMID:31604608^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Acheson JF, Derewenda ZS, Zimmer J. Architecture of the Cellulose Synthase Outer Membrane Channel and Its Association with the Periplasmic TPR Domain. Structure. 2019 Oct 1. pii: S0969-2126(19)30314-4. doi:, 10.1016/j.str.2019.09.008. PMID:31604608 doi:https://dx.doi.org/10.1016/j.str.2019.09.008

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OCA

[1] Acheson JF, Derewenda ZS, Zimmer J. Architecture of the Cellulose Synthase Outer Membrane Channel and Its Association with the Periplasmic TPR Domain. Structure. 2019 Oct 1. pii: S0969-2126(19)30314-4. doi:, 10.1016/j.str.2019.09.008. PMID:31604608 doi:https://dx.doi.org/10.1016/j.str.2019.09.008

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='6tzk' size='340' side='right'caption='[[6tzk]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6tzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TZK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6tzk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TZK FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bcsC, yhjL, b3530, JW5942 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tzk OCA], [https://pdbe.org/6tzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tzk RCSB], [https://www.ebi.ac.uk/pdbsum/6tzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tzk ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tzk OCA], [http://pdbe.org/6tzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tzk RCSB], [http://www.ebi.ac.uk/pdbsum/6tzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tzk ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BCSC_ECOLI BCSC_ECOLI]] Required for maximal bacterial cellulose synthesis.
+[https://www.uniprot.org/uniprot/BCSC_ECOLI BCSC_ECOLI] Required for maximal bacterial cellulose synthesis.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Acheson, J F]]
+[[Category: Acheson JF]]
-[[Category: Derewenda, Z]]
+[[Category: Derewenda Z]]
-[[Category: Zimmer, J]]
+[[Category: Zimmer J]]
-[[Category: Cellulose synthase]]
-[[Category: Membrane protein]]
-[[Category: Polysaccharide secretion]]
-[[Category: Porin]]

6tzk: Difference between revisions

Latest revision as of 10:32, 15 February 2023

Bacterial cellulose synthase outermembrane channel BcsC with terminal TPR repeat

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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