1a67: Difference between revisions

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[[Image:1a67.gif|left|200px]]
{{Seed}}
[[Image:1a67.png|left|200px]]


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{{STRUCTURE_1a67|  PDB=1a67  |  SCENE=  }}  
{{STRUCTURE_1a67|  PDB=1a67  |  SCENE=  }}  


'''CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES'''
===CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES===




==Overview==
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The solution structures of the phosphorylated form of native chicken cystatin and the recombinant variant AEF-S1M-M29I-M89L were determined by 2D, 3D and 4D-NMR. The structures turn out to be very similar, despite the substitutions and the phosphorylation of the wild-type. Their dominant feature is a five-stranded beta-sheet, which is wrapped around a five-turn alpha-helix, as shown by X-ray crystallographic studies of wild-type chicken cystatin. However, the NMR analysis shows that the second helix observed in the crystal is not present in solution. The phosphorylation occurs at S80, which is located in a flexible region. For this reason, very few effects on the structure are observed. Comparison of structures of the unphosphorylated variant and the wild-type shows small effects on H84 which is located in the supposed recognition site of the serine kinase. This recognition site appears to be well structured as a large loop-containing bulge of the beta-sheet. The N termini of both mutants, which contribute to a large extent to the binding to the proteinase, are very flexible. A loop structure involving the residues L7 to A10 as found in related inhibitors, such as in the kininogen domains 2 and 3, is not sufficiently populated to be observed.
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{{ABSTRACT_PUBMED_8263912}}


==About this Structure==
==About this Structure==
1A67 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA].  
1A67 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA].  


==Reference==
==Reference==
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[[Category: Steffin]]
[[Category: Steffin]]
[[Category: Thiol proteinase]]
[[Category: Thiol proteinase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:52:23 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:13:34 2008''

Revision as of 13:13, 30 June 2008

File:1a67.png

Template:STRUCTURE 1a67

CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES

Template:ABSTRACT PUBMED 8263912

About this Structure

1A67 is a Single protein structure of sequence from Gallus gallus. Full experimental information is available from OCA.

Reference

The structures of native phosphorylated chicken cystatin and of a recombinant unphosphorylated variant in solution., Dieckmann T, Mitschang L, Hofmann M, Kos J, Turk V, Auerswald EA, Jaenicke R, Oschkinat H, J Mol Biol. 1993 Dec 20;234(4):1048-59. PMID:8263912

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