4gft

Malaria invasion machinery protein-Nanobody complex

spinqualitylabelsall models 4gft, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4gft is a 2 chain structure with sequence from Lama glama and Plasmodium falciparum 3D7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, PCA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8I4W8_PLAF7 Publication Abstract from PubMed Apicomplexan parasites enter host cells by many sophisticated steps including use of an ATP-powered invasion machinery. The machinery consists of multiple proteins, including a special myosin (MyoA) which moves along an actin fiber and which is connected to the myosin tail interaction protein (MTIP). Here we report a crystal structure of the major MyoA-binding domain (D3) of Plasmodium falciparum MTIP in complex with an anti-MTIP nanobody. In this complex, the MyoA-binding groove in MTIP-D3 is considerably less accessible than when occupied by the MyoA helix, due to a shift of two helices. The nanobody binds to an area slightly overlapping with the MyoA binding groove, covering a hydrophobic region next to the groove entrance. This provides a new avenue for arriving at compounds interfering with the invasion machinery since small molecules binding simultaneously to the nanobody binding site and the adjacent MyoA binding groove would prevent MyoA binding by MTIP. The structure of the D3 domain of Plasmodium falciparum myosin tail interacting protein MTIP in complex with a nanobody.,Khamrui S, Turley S, Pardon E, Steyaert J, Fan E, Verlinde CL, Bergman LW, Hol WG Mol Biochem Parasitol. 2013 Aug;190(2):87-91. doi:, 10.1016/j.molbiopara.2013.06.003. Epub 2013 Jul 4. PMID:23831371 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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