1a70: Difference between revisions

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[[Image:1a70.gif|left|200px]]
{{Seed}}
[[Image:1a70.png|left|200px]]


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{{STRUCTURE_1a70|  PDB=1a70  |  SCENE=  }}  
{{STRUCTURE_1a70|  PDB=1a70  |  SCENE=  }}  


'''SPINACH FERREDOXIN'''
===SPINACH FERREDOXIN===




==Overview==
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Ferredoxin I (Fd I) from Spinacia oleracea is composed of 97 amino-acid residues and a [2Fe-2S] cluster. The crystal structure of the E92K mutant of Fd I was solved by molecular replacement and refined to an R factor of 19.6% for 11755 reflections at 1.7 A resolution. The overall structure and the active centre of spinach Fd is highly conserved with respect to ferredoxins of known structure. The E92K mutation appears to disturb a hydrogen-bond network which stabilizes the loop bearing the [2Fe-2S] cluster. This observation provides a rationale for the reduced electron-transfer efficiency displayed by the E92K mutant. Inspection of the crystal packing reveals that the side chain of Lys92 is engaged in an intermolecular interaction with Asp26 of a symmetry-related molecule. This feature may explain why only the mutant E92K and not wild-type Fd I could be successfully crystallized.
The line below this paragraph, {{ABSTRACT_PUBMED_10089511}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10089511 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10089511}}


==About this Structure==
==About this Structure==
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[[Category: Iron-sulfur protein]]
[[Category: Iron-sulfur protein]]
[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:54:32 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:16:46 2008''

Revision as of 13:16, 30 June 2008

File:1a70.png

Template:STRUCTURE 1a70

SPINACH FERREDOXIN

Template:ABSTRACT PUBMED 10089511

About this Structure

1A70 is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.

Reference

Structure of the mutant E92K of [2Fe-2S] ferredoxin I from Spinacia oleracea at 1.7 A resolution., Binda C, Coda A, Aliverti A, Zanetti G, Mattevi A, Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1353-8. PMID:10089511

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