1a73: Difference between revisions

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[[Image:1a73.gif|left|200px]]
{{Seed}}
[[Image:1a73.png|left|200px]]


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{{STRUCTURE_1a73|  PDB=1a73  |  SCENE=  }}  
{{STRUCTURE_1a73|  PDB=1a73  |  SCENE=  }}  


'''INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA'''
===INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA===




==Overview==
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Homing endonucleases are a diverse collection of proteins that are encoded by genes with mobile, self-splicing introns. They have also been identified in self-splicing inteins (protein introns). These enzymes promote the movement of the DNA sequences that encode them from one chromosome location to another; they do this by making a site-specific double-strand break at a target site in an allele that lacks the corresponding mobile intron. The target sites recognized by these small endonucleases are generally long (14-44 base pairs). Four families of homing endonucleases have been identified, including the LAGLIDADG, the His-Cys box, the GIY-YIG and the H-N-H endonucleases. The first identified His-Cys box homing endonuclease was I-PpoI from the slime mould Physarum polycephalum. Its gene resides in one of only a few nuclear introns known to exhibit genetic mobility. Here we report the structure of the I-PpoI homing endonuclease bound to homing-site DNA determined to 1.8 A resolution. I-PpoI displays an elongated fold of dimensions 25 x 35 x 80 A, with mixed alpha/beta topology. Each I-PpoI monomer contains three antiparallel beta-sheets flanked by two long alpha-helices and a long carboxy-terminal tail, and is stabilized by two bound zinc ions 15 A apart. The enzyme possesses a new zinc-bound fold and endonuclease active site. The structure has been determined in both uncleaved substrate and cleaved product complexes.
The line below this paragraph, {{ABSTRACT_PUBMED_9665136}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9665136 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9665136}}


==About this Structure==
==About this Structure==
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[[Category: Protein folding]]
[[Category: Protein folding]]
[[Category: Zinc]]
[[Category: Zinc]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:54:47 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:17:06 2008''

Revision as of 13:17, 30 June 2008

File:1a73.png

Template:STRUCTURE 1a73

INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA

Template:ABSTRACT PUBMED 9665136

About this Structure

1A73 is a Single protein structure of sequence from Physarum polycephalum. Full crystallographic information is available from OCA.

Reference

DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI., Flick KE, Jurica MS, Monnat RJ Jr, Stoddard BL, Nature. 1998 Jul 2;394(6688):96-101. PMID:9665136

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