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==HnRNP A1 Alters the Conformation of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation==
==HnRNP A1 Alters the Conformation of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation==
<StructureSection load='5v17' size='340' side='right'caption='[[5v17]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='5v17' size='340' side='right'caption='[[5v17]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5v17]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V17 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5v17]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_A71 Enterovirus A71]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V17 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5v16|5v16]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v17 OCA], [http://pdbe.org/5v17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v17 RCSB], [http://www.ebi.ac.uk/pdbsum/5v17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v17 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v17 OCA], [https://pdbe.org/5v17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v17 RCSB], [https://www.ebi.ac.uk/pdbsum/5v17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v17 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enteroviruses use a type I Internal Ribosome Entry Site (IRES) structure to facilitate protein synthesis and promote genome replication. Type I IRES elements require auxiliary host proteins to organize RNA structure for 40S ribosomal subunit assembly. Heterogeneous nuclear ribonucleoprotein A1 stimulates enterovirus 71 (EV71) translation in part through specific interactions with its stem loop II (SLII) IRES domain. Here, we determined a conjoined NMR-small angle x-ray scattering structure of the EV71 SLII domain and a mutant that significantly attenuates viral replication by abrogating hnRNP A1 interactions. Native SLII adopts a locally compact structure wherein stacking interactions in a conserved 5'-AUAGC-3' bulge preorganize the adjacent helices at nearly orthogonal orientations. Mutating the bulge sequence to 5'-ACCCC-3' ablates base stacking in the loop and globally reorients the SLII structure. Biophysical titrations reveal that the 5'-AUAGC-3' bulge undergoes a conformational change to assemble a functional hnRNP A1-RNA complex. Importantly, IRES mutations that delete the bulge impair viral translation and completely inhibit replication. Thus, this work provides key details into how an EV71 IRES structure adapts to hijack a cellular protein, and it suggests that the SLII domain is a potential target for antiviral therapy.
HnRNP A1 Alters the Structure of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation.,Tolbert M, Morgan CE, Pollum M, Crespo-Hernandez CE, Li ML, Brewer G, Tolbert BS J Mol Biol. 2017 Jun 15. pii: S0022-2836(17)30306-6. doi:, 10.1016/j.jmb.2017.06.007. PMID:28625847<ref>PMID:28625847</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5v17" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Enterovirus A71]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Morgan, C E]]
[[Category: Morgan CE]]
[[Category: Tolbert, B S]]
[[Category: Tolbert BS]]
[[Category: Tolbert, M]]
[[Category: Tolbert M]]
[[Category: Rna]]

Latest revision as of 07:28, 1 May 2024

HnRNP A1 Alters the Conformation of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation

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