6o0w: Difference between revisions

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<StructureSection load='6o0w' size='340' side='right'caption='[[6o0w]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
<StructureSection load='6o0w' size='340' side='right'caption='[[6o0w]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6o0w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Muscadine_grape Muscadine grape]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O0W FirstGlance]. <br>
<table><tr><td colspan='2'>[[6o0w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vitis_rotundifolia Vitis rotundifolia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O0W FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2P:ADENOSINE-2-5-DIPHOSPHATE'>A2P</scene>, <scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RUN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103349 Muscadine grape])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2P:ADENOSINE-2-5-DIPHOSPHATE'>A2P</scene>, <scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0w OCA], [http://pdbe.org/6o0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o0w RCSB], [http://www.ebi.ac.uk/pdbsum/6o0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0w ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0w OCA], [https://pdbe.org/6o0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o0w RCSB], [https://www.ebi.ac.uk/pdbsum/6o0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0w ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/RUN1_VITRO RUN1_VITRO]] Disease resistance (R) protein that confers resistance to multiple powdery and downy mildew by promoting cell death (PubMed:24033846, PubMed:31439792). Acts as a NAD(+) hydrolase (NADase): in response to activation, catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR) and nicotinamide; NAD(+) cleavage triggering a defense system that promotes cell death (PubMed:31439792). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (PubMed:31439792).<ref>PMID:24033846</ref> <ref>PMID:31439792</ref> 
[https://www.uniprot.org/uniprot/RUN1_VITRO RUN1_VITRO] Disease resistance (R) protein that confers resistance to multiple powdery and downy mildew by promoting cell death (PubMed:24033846, PubMed:31439792). Acts as a NAD(+) hydrolase (NADase): in response to activation, catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR) and nicotinamide; NAD(+) cleavage triggering a defense system that promotes cell death (PubMed:31439792). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (PubMed:31439792).<ref>PMID:24033846</ref> <ref>PMID:31439792</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
SARM1 (sterile alpha and TIR motif containing 1) is responsible for depletion of nicotinamide adenine dinucleotide in its oxidized form (NAD(+)) during Wallerian degeneration associated with neuropathies. Plant nucleotide-binding leucine-rich repeat (NLR) immune receptors recognize pathogen effector proteins and trigger localized cell death to restrict pathogen infection. Both processes depend on closely related Toll/interleukin-1 receptor (TIR) domains in these proteins, which, as we show, feature self-association-dependent NAD(+) cleavage activity associated with cell death signaling. We further show that SARM1 SAM (sterile alpha motif) domains form an octamer essential for axon degeneration that contributes to TIR domain enzymatic activity. The crystal structures of ribose and NADP(+) (the oxidized form of nicotinamide adenine dinucleotide phosphate) complexes of SARM1 and plant NLR RUN1 TIR domains, respectively, reveal a conserved substrate binding site. NAD(+) cleavage by TIR domains is therefore a conserved feature of animal and plant cell death signaling pathways.
 
NAD(+) cleavage activity by animal and plant TIR domains in cell death pathways.,Horsefield S, Burdett H, Zhang X, Manik MK, Shi Y, Chen J, Qi T, Gilley J, Lai JS, Rank MX, Casey LW, Gu W, Ericsson DJ, Foley G, Hughes RO, Bosanac T, von Itzstein M, Rathjen JP, Nanson JD, Boden M, Dry IB, Williams SJ, Staskawicz BJ, Coleman MP, Ve T, Dodds PN, Kobe B Science. 2019 Aug 23;365(6455):793-799. doi: 10.1126/science.aax1911. PMID:31439792<ref>PMID:31439792</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6o0w" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Muscadine grape]]
[[Category: Vitis rotundifolia]]
[[Category: Boden, M]]
[[Category: Boden M]]
[[Category: Bosanac, T]]
[[Category: Bosanac T]]
[[Category: Burdett, H]]
[[Category: Burdett H]]
[[Category: Casey, L]]
[[Category: Casey L]]
[[Category: Chen, J]]
[[Category: Chen J]]
[[Category: Coleman, M P]]
[[Category: Coleman MP]]
[[Category: Dodds, P N]]
[[Category: Dodds PN]]
[[Category: Dry, I B]]
[[Category: Dry IB]]
[[Category: Ericsson, D J]]
[[Category: Ericsson DJ]]
[[Category: Foley, G]]
[[Category: Foley G]]
[[Category: Gilley, J]]
[[Category: Gilley J]]
[[Category: Gu, W]]
[[Category: Gu W]]
[[Category: Horsefield, S]]
[[Category: Horsefield S]]
[[Category: Hughes, R O]]
[[Category: Hughes RO]]
[[Category: Itzstein, M von]]
[[Category: Kobe B]]
[[Category: Kobe, B]]
[[Category: Lai J]]
[[Category: Lai, J]]
[[Category: Manik MK]]
[[Category: Manik, M K]]
[[Category: Nanson JD]]
[[Category: Nanson, J D]]
[[Category: Rank M]]
[[Category: Rank, M]]
[[Category: Rathjen JP]]
[[Category: Rathjen, J P]]
[[Category: Shi Y]]
[[Category: Shi, Y]]
[[Category: Staskawicz BJ]]
[[Category: Staskawicz, B J]]
[[Category: Tiancong Q]]
[[Category: Tiancong, Q]]
[[Category: Ve T]]
[[Category: Ve, T]]
[[Category: Williams SJ]]
[[Category: Williams, S J]]
[[Category: Zhang X]]
[[Category: Zhang, X]]
[[Category: Von Itzstein M]]
[[Category: Plant disease resistance]]
[[Category: Signaling protein]]

Revision as of 14:50, 13 March 2024

Crystal structure of the TIR domain from the grapevine disease resistance protein RUN1 in complex with NADP+ and Bis-Tris

6o0w, resolution 1.75Å

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