6ttk: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6ttk ~~is ON HOLD~~

==Crystal structure of the kelch domain of human KLHL12 in complex with DVL1 peptide==

~~Authors~~: ~~Chen, Z~~.~~, Williams, E~~.~~, Pike, A~~.C.W., ~~Strain~~-~~Damerell~~, C.~~, Wang, D~~.~~, Chalk, R~~.~~, Burgess~~-~~Brown~~, N., ~~Krojer, T~~., ~~von Delft, F~~.~~, Arrowsmith, C~~.H., ~~Edwards, A~~.M.~~, Bountra, C~~., ~~Bullock, A~~.N.

== Structural highlights ==

<table><tr><td colspan='2'>[[6ttk]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TTK FirstGlance]. <br>

~~Description~~: ~~Crystal structure~~ of the ~~kelch domain~~ of ~~human~~ KLHL12 in complex ~~with DVL1 peptide~~

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>

[[Category: ~~Unreleased~~ Structures]]

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ttk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ttk OCA], [http://pdbe.org/6ttk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ttk RCSB], [http://www.ebi.ac.uk/pdbsum/6ttk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ttk ProSAT]</span></td></tr>

[[Category: ~~Pike~~, A.C.W]]

</table>

[[Category: ~~Arrowsmith~~, C.H]]

== Function ==

[[http://www.uniprot.org/uniprot/KLH12_HUMAN KLH12_HUMAN]] Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export (PubMed:27716508). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed:16547521). The BCR(KLHL12) complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins (PubMed:18303015, PubMed:20100572, PubMed:27716508).<ref>PMID:16547521</ref> <ref>PMID:18303015</ref> <ref>PMID:20100572</ref> <ref>PMID:22358839</ref> <ref>PMID:27565346</ref> <ref>PMID:27716508</ref>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Arrowsmith, C H]]

[[Category: Bountra, C]]

[[Category: Bullock, A N]]

[[Category: Burgess-Brown, N]]

[[Category: Chalk, R]]

~~[[Category: Williams, E]]~~

~~[[Category: Wang, D]]~~

~~[[Category: Von Delft, F]]~~

~~[[Category: Bullock, A.N]]~~

~~[[Category: Edwards, A.M]]~~

[[Category: Chen, Z]]

[[Category: Delft, F von]]

[[Category: Edwards, A M]]

[[Category: Krojer, T]]

[[Category: Pike, A C.W]]

[[Category: Strain-Damerell, C]]

[[Category: ~~Krojer~~, T]]

[[Category: Wang, D]]

[[Category: ~~Bountra~~, C]]

[[Category: Williams, E]]

[[Category: Complex]]

[[Category: Cullin3 e3 ligase]]

[[Category: Kelch]]

[[Category: Ligase]]

[[Category: Substrate peptide]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6ttk is ON HOLD
+==Crystal structure of the kelch domain of human KLHL12 in complex with DVL1 peptide==
+<StructureSection load='6ttk' size='340' side='right'caption='[[6ttk]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
-Authors: Chen, Z., Williams, E., Pike, A.C.W., Strain-Damerell, C., Wang, D., Chalk, R., Burgess-Brown, N., Krojer, T., von Delft, F., Arrowsmith, C.H., Edwards, A.M., Bountra, C., Bullock, A.N.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6ttk]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TTK FirstGlance]. <br>
-Description: Crystal structure of the kelch domain of human KLHL12 in complex with DVL1 peptide
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ttk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ttk OCA], [http://pdbe.org/6ttk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ttk RCSB], [http://www.ebi.ac.uk/pdbsum/6ttk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ttk ProSAT]</span></td></tr>
-[[Category: Pike, A.C.W]]
+</table>
-[[Category: Arrowsmith, C.H]]
+== Function ==
+[[http://www.uniprot.org/uniprot/KLH12_HUMAN KLH12_HUMAN]] Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export (PubMed:27716508). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed:16547521). The BCR(KLHL12) complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins (PubMed:18303015, PubMed:20100572, PubMed:27716508).<ref>PMID:16547521</ref> <ref>PMID:18303015</ref> <ref>PMID:20100572</ref> <ref>PMID:22358839</ref> <ref>PMID:27565346</ref> <ref>PMID:27716508</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Arrowsmith, C H]]
+[[Category: Bountra, C]]
+[[Category: Bullock, A N]]
 [[Category: Burgess-Brown, N]]
 [[Category: Chalk, R]]
-[[Category: Williams, E]]
-[[Category: Wang, D]]
-[[Category: Von Delft, F]]
-[[Category: Bullock, A.N]]
-[[Category: Edwards, A.M]]
 [[Category: Chen, Z]]
+[[Category: Delft, F von]]
+[[Category: Edwards, A M]]
+[[Category: Krojer, T]]
+[[Category: Pike, A C.W]]
 [[Category: Strain-Damerell, C]]
-[[Category: Krojer, T]]
+[[Category: Wang, D]]
-[[Category: Bountra, C]]
+[[Category: Williams, E]]
+[[Category: Complex]]
+[[Category: Cullin3 e3 ligase]]
+[[Category: Kelch]]
+[[Category: Ligase]]
+[[Category: Substrate peptide]]