Sandbox Reserved 1091: Difference between revisions
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A peptide can be inserted in the space of the active site. There, the amino acids of the catalytic triad will interact together and the mechanism will lead to a cut in the polypeptide. | A peptide can be inserted in the space of the active site. There, the amino acids of the catalytic triad will interact together and the mechanism will lead to a cut in the polypeptide. | ||
The mechanism is the following: The histidine will react with the Serine and deprotonate it. The deprotonated hydroxyl group of the serine will act as a nucleophilic species and attack the carbon from the carbonyl function on the peptide. This will lead to the formation of a tetrahedral intermediate. At the end, the regeneration of the active site will be done with the release of the peptide cut in two parts. | <b> Mechanism: </b>The mechanism is the following: The histidine will react with the Serine and deprotonate it. The deprotonated hydroxyl group of the serine will act as a nucleophilic species and attack the carbon from the carbonyl function on the peptide. This will lead to the formation of a tetrahedral intermediate. At the end, the regeneration of the active site will be done with the release of the peptide cut in two parts. | ||
