Sandbox Reserved 1096: Difference between revisions

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==='''Catalysis of deimination'''===

== Citrullination of Arginine residues ==

In humans, PAD2 is involved in a type of post-translational modification called citrullination. Indeed, this calcium-dependent enzyme catalyzes a deimination reaction : PAD2 uses one molecule of water to replace the terminal nitrogen of Arginine by an oxygen and a ketone group is formed in place of a ketimine one.

The transformation of arginyl residues ([https://fr.wikipedia.org/wiki/Arginine Arginine]), which are positively charged, into citrullyl residues, which are neutral, leads to the modification of the global charge of the targeted protein. These residue modifications are the result of hydrolysation of guanidinium groups in side chains and may produce important conformational changes in proteins by increasing the hydrophobicity. The catalytic residue which allows hydrolyzation of the guanidium group is a '''''nucleophilic cysteine in position 647'''''. The proper positioning of C647 is due to the binding of calcium ions to PAD 2, essential for deamination reaction.

The transformation of arginyl residues ([https://fr.wikipedia.org/wiki/Arginine Arginine]), which are positively charged, into citrullyl residues, which are neutral, leads to the '''''modification of the global charge''''' of the targeted protein. These residue modifications are the result of hydrolysation of guanidinium groups in side chains and may produce important conformational changes in proteins by increasing the hydrophobicity. The catalytic residue which allows hydrolyzation of the guanidium group is a '''''nucleophilic cysteine in position 647'''''. The proper positioning of C647 is due to the binding of calcium ions to PAD 2, essential for deamination reaction.

Sandbox Reserved 1096: Difference between revisions

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@@ Line 18: / Line 18: @@
 ==='''Catalysis of deimination'''===
 == Citrullination of Arginine residues ==
 In humans, PAD2 is involved in a type of post-translational modification called citrullination. Indeed, this calcium-dependent enzyme catalyzes a deimination reaction : PAD2 uses one molecule of water to replace the terminal nitrogen of Arginine by an oxygen and a ketone group is formed in place of a ketimine one.
-The transformation of arginyl residues ([https://fr.wikipedia.org/wiki/Arginine Arginine]), which are positively charged, into citrullyl residues, which are neutral, leads to the modification of the global charge of the targeted protein. These residue modifications are the result of hydrolysation of guanidinium groups in side chains and may produce important conformational changes in proteins by increasing the hydrophobicity. The catalytic residue which allows hydrolyzation of the guanidium group is a '''''nucleophilic cysteine in position 647'''''. The proper positioning of C647 is due to the binding of calcium ions to PAD 2, essential for deamination reaction.
+The transformation of arginyl residues ([https://fr.wikipedia.org/wiki/Arginine Arginine]), which are positively charged, into citrullyl residues, which are neutral, leads to the '''''modification of the global charge''''' of the targeted protein. These residue modifications are the result of hydrolysation of guanidinium groups in side chains and may produce important conformational changes in proteins by increasing the hydrophobicity. The catalytic residue which allows hydrolyzation of the guanidium group is a '''''nucleophilic cysteine in position 647'''''. The proper positioning of C647 is due to the binding of calcium ions to PAD 2, essential for deamination reaction.