Sandbox Reserved 1106: Difference between revisions
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==== Primary Structure ==== | ==== Primary Structure ==== | ||
The Ago1 protein has the same 4 primary domains as all argonaut (N, PAZ, Mid, PIWI, describe in the page [ | The Ago1 protein has the same 4 primary domains as all argonaut (N, PAZ, Mid, PIWI, describe in the page [http://proteopedia.org/wiki/index.php/Argonaute]) and the two linker regions L1 (also called DUF1785 domain) and L2. Indeed, there is 84% of similitudes between the primary sequence of hAgo1 and hAgo2. Besides the N domain is interacting with L1, L2 and PIWI domains via residues 18-48 and 138-173. | ||
Besides, Argonaut 1 can be found in a lot of phylogenetic groups, with different structure. For example, in the plant ''Brachypodium distachyon'', where 10 argonauts proteins have been discovered, BdAGO1 lacks the N and Mid domains. This particularity explains the small size of this protein with only 624 residues. Moreover, BdAGO1 is related to Ago1 since the catalytic tetrad in the PIWI domain doesn’t work for it, enabling an endonuclease activity due to a missing of the last D/H residue | Besides, Argonaut 1 can be found in a lot of phylogenetic groups, with different structure. For example, in the plant ''Brachypodium distachyon'', where 10 argonauts proteins have been discovered, BdAGO1 lacks the N and Mid domains. This particularity explains the small size of this protein with only 624 residues. Moreover, BdAGO1 is related to Ago1 since the catalytic tetrad in the PIWI domain doesn’t work for it, enabling an endonuclease activity due to a missing of the last D/H residue | ||
