Sandbox Reserved 1101: Difference between revisions

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==Generalities on fiber assembly of dragline silks==

The process of the dragline fiber formation is the connection of '''soluble MaSp''' proteins into '''insoluble fibers'''. Indeed, MaSps are firstly secreted and stored in soluble form in the tail of the major ampullate gland which is located in the spider’s abdomen. On demand, they pass through the narrow duct where they experience mechanical and chemical forces that convert them into fibers. Actually, they deal with a pH dropping, an alteration of ion concentrations and oxidation conditions, which occur gradually along the duct. These changes promote the connection of MaSps extremities (i.e. homo-dimerisation of C- and N-terminal domains) to form fibers. Finally, through flow rate and mechanical forces experienced in the duct, the fibers will agglomerate to create the '''dragline silk'''.

The process of the dragline fiber formation is the connection of '''soluble MaSp''' proteins into '''insoluble fibers'''. Indeed, MaSps are firstly secreted and stored in soluble form in the tail of the major ampullate gland which is located in the spider’s abdomen. On demand, they pass through the narrow duct where they experience mechanical and chemical forces that convert them into fibers.

[[Image:Image:Major ampullate gland.jpeg|300px|center|thumb| Schematic of Major ampullate gland of spiders. From Andersson et al. (2013). Morphology and composition of the spider major ampullate gland and dragline silk. Biomacromolecules ''14'', 2945-2952.]]

Actually, they deal with a pH dropping, an alteration of ion concentrations and oxidation conditions, which occur gradually along the duct. These changes promote the connection of MaSps extremities (i.e. homo-dimerisation of C- and N-terminal domains) to form fibers. Finally, through flow rate and mechanical forces experienced in the duct, the fibers will agglomerate to create the '''dragline silk'''.

[[Image:MaSp assembly.jpg|300px|center|thumb| Model of MaSps assembly according to the pH conditions. From Gaines et al. (2010). Spidroin N-terminal domain promotes a pH-dependent association of silk proteins during self-assembly. J. Biol. Chem. ''285'', 40745-40753.]]

Sandbox Reserved 1101: Difference between revisions

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@@ Line 12: / Line 12: @@
 ==Generalities on fiber assembly of dragline silks==
-The process of the dragline fiber formation is the connection of '''soluble MaSp''' proteins into '''insoluble fibers'''. Indeed, MaSps are firstly secreted and stored in soluble form in the tail of the major ampullate gland which is located in the spider’s abdomen. On demand, they pass through the narrow duct where they experience mechanical and chemical forces that convert them into fibers. Actually, they deal with a pH dropping, an alteration of ion concentrations and oxidation conditions, which occur gradually along the duct. These changes promote the connection of MaSps extremities (i.e. homo-dimerisation of C- and N-terminal domains) to form fibers. Finally, through flow rate and mechanical forces experienced in the duct, the fibers will agglomerate to create the '''dragline silk'''.
+The process of the dragline fiber formation is the connection of '''soluble MaSp''' proteins into '''insoluble fibers'''. Indeed, MaSps are firstly secreted and stored in soluble form in the tail of the major ampullate gland which is located in the spider’s abdomen. On demand, they pass through the narrow duct where they experience mechanical and chemical forces that convert them into fibers.
+[[Image:Image:Major ampullate gland.jpeg|300px|center|thumb| Schematic of Major ampullate gland of spiders. From Andersson et al. (2013). Morphology and composition of the spider major ampullate gland and dragline silk. Biomacromolecules ''14'', 2945-2952.]]
+Actually, they deal with a pH dropping, an alteration of ion concentrations and oxidation conditions, which occur gradually along the duct. These changes promote the connection of MaSps extremities (i.e. homo-dimerisation of C- and N-terminal domains) to form fibers. Finally, through flow rate and mechanical forces experienced in the duct, the fibers will agglomerate to create the '''dragline silk'''.
+[[Image:MaSp assembly.jpg|300px|center|thumb| Model of MaSps assembly according to the pH conditions. From Gaines et al. (2010). Spidroin N-terminal domain promotes a pH-dependent association of silk proteins during self-assembly. J. Biol. Chem. ''285'', 40745-40753.]]