1acc: Difference between revisions

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[[Image:1acc.gif|left|200px]]
[[Image:1acc.png|left|200px]]


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==Overview==
{{ABSTRACT_9039918}}
Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax. After proteolytic activation on the host cell surface, PA forms a membrane-inserting heptamer that translocates the toxic enzymes, oedema factor and lethal factor, into the cytosol. PA, which has a relative molecular mass of 83,000 (M(r) 83K), can also translocate heterologous proteins, and is being evaluated for use as a general protein delivery system. Here we report the crystal structure of monomeric PA at 2.1 A resolution and the water-soluble heptamer at 4.5 A resolution. The monomer is organized mainly into antiparallel beta-sheets and has four domains: an amino-terminal domain (domain 1) containing two calcium ions and the cleavage site for activating proteases; a heptamerization domain (domain 2) containing a large flexible loop implicated in membrane insertion; a small domain of unknown function (domain 3); and a carboxy-terminal receptor-binding domain (domain 4). Removal of a 20K amino-terminal fragment from domain 1 allows the assembly of the heptamer, a ring-shaped structure with a negatively charged lumen, and exposes a large hydrophobic surface for binding the toxic enzymes. We propose a model of pH-dependent membrane insertion involving the formation of a porin-like, membrane-spanning beta-barrel.


==About this Structure==
==About this Structure==
1ACC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The following page contains interesting information on the relation of 1ACC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb28_1.html Anthrax Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACC OCA].  
1ACC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The following page contains interesting information on the relation of 1ACC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb28_1.html Anthrax Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACC OCA].  
==Reference==
Crystal structure of the anthrax toxin protective antigen., Petosa C, Collier RJ, Klimpel KR, Leppla SH, Liddington RC, Nature. 1997 Feb 27;385(6619):833-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9039918 9039918]
[[Category: Anthrax Toxin]]
[[Category: Anthrax Toxin]]
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
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[[Category: Calcium-binding]]
[[Category: Calcium-binding]]
[[Category: Toxin]]
[[Category: Toxin]]
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