6vhb: Difference between revisions
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The | ==1.4A low-dose structure of GSNQNNF determined from initial phases generated using radiation damage== | ||
<StructureSection load='6vhb' size='340' side='right'caption='[[6vhb]], [[Resolution|resolution]] 1.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6vhb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6VHB FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6cli|6cli]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6vhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vhb OCA], [http://pdbe.org/6vhb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vhb RCSB], [http://www.ebi.ac.uk/pdbsum/6vhb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vhb ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to determine atomic-resolution structures from vanishingly small three-dimensional crystals. Here, we present an example of an experimentally phased structure using only MicroED data. The structure of a seven-residue peptide is solved starting from differences to the diffraction intensities induced by structural changes due to radiation damage. The same wedge of reciprocal space was recorded twice by continuous-rotation MicroED from a set of 11 individual crystals. The data from the first pass were merged to make a "low-dose dataset." The data from the second pass were similarly merged to form a "damaged dataset." Differences between these two datasets were used to identify a single heavy-atom site from a Patterson difference map, and initial phases were generated. Finally, the structure was completed by iterative cycles of modeling and refinement. | |||
Experimental Phasing of MicroED Data Using Radiation Damage.,Martynowycz MW, Hattne J, Gonen T Structure. 2020 Feb 4. pii: S0969-2126(20)30008-3. doi:, 10.1016/j.str.2020.01.008. PMID:32023481<ref>PMID:32023481</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6vhb" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Gonen, T]] | |||
[[Category: Hattne, J]] | |||
[[Category: Martynowycz, M W]] | |||
[[Category: Damage]] | |||
[[Category: Microed]] | |||
[[Category: Phasing]] | |||
[[Category: Protein fibril]] | |||
[[Category: Rip]] | |||
Revision as of 06:44, 19 February 2020
1.4A low-dose structure of GSNQNNF determined from initial phases generated using radiation damage
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