6vhc: Difference between revisions

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'''Unreleased structure'''


The entry 6vhc is ON HOLD
==1.4A damaged structure of GSNQNNF used to determine initial phases from radiation damage==
<StructureSection load='6vhc' size='340' side='right'caption='[[6vhc]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6vhc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6VHC FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6clj|6clj]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6vhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vhc OCA], [http://pdbe.org/6vhc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vhc RCSB], [http://www.ebi.ac.uk/pdbsum/6vhc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vhc ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to determine atomic-resolution structures from vanishingly small three-dimensional crystals. Here, we present an example of an experimentally phased structure using only MicroED data. The structure of a seven-residue peptide is solved starting from differences to the diffraction intensities induced by structural changes due to radiation damage. The same wedge of reciprocal space was recorded twice by continuous-rotation MicroED from a set of 11 individual crystals. The data from the first pass were merged to make a "low-dose dataset." The data from the second pass were similarly merged to form a "damaged dataset." Differences between these two datasets were used to identify a single heavy-atom site from a Patterson difference map, and initial phases were generated. Finally, the structure was completed by iterative cycles of modeling and refinement.


Authors:  
Experimental Phasing of MicroED Data Using Radiation Damage.,Martynowycz MW, Hattne J, Gonen T Structure. 2020 Feb 4. pii: S0969-2126(20)30008-3. doi:, 10.1016/j.str.2020.01.008. PMID:32023481<ref>PMID:32023481</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 6vhc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Gonen, T]]
[[Category: Hattne, J]]
[[Category: Martynowycz, M W]]
[[Category: Damage]]
[[Category: Microed]]
[[Category: Phasing]]
[[Category: Protein fibril]]
[[Category: Rip]]
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