6sd0

Structure of beta-galactosidase from Thermotoga maritima.

spinqualitylabelsall models 6sd0, resolution 3.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6sd0 is a 4 chain structure with sequence from Thermotoga maritima MSB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.7Å Ligands: MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BGAL_THEMA Publication Abstract from PubMed Lactose intolerance is a common digestive disorder that affects a large proportion of the adult human population. The severity of the symptoms is highly variable, depending on the susceptibility to the sugar and the amount digested. For that reason, enzymes that can be used for the production of lactose-free milk and milk derivatives have acquired singular biotechnological importance. One such case is Thermotoga maritima beta-galactosidase (TmLac). Here, we report the cryo-EM structure of TmLac at 2.0 A resolution. The protein features a newly solved domain at its C-terminus, characteristic of the genus Thermotoga, which promotes a peculiar octameric arrangement. We have assessed the constraints imposed by the quaternary protein structure on the construction of hybrid versions of this GH2 enzyme. Carbohydrate binding modules (CBM) from the CBM2 and CBM9 families have been added at either the amino or carboxy terminus, and the structural and functional effects of such modifications have been analyzed. The results provide a basis for the rational design of hybrid enzymes that can be efficiently attached to different solid supports. The cryo-EM Structure of Thermotoga maritima beta-Galactosidase: Quaternary Structure Guides Protein Engineering.,Miguez Amil S, Jimenez-Ortega E, Ramirez-Escudero M, Talens-Perales D, Marin-Navarro J, Polaina J, Sanz-Aparicio J, Fernandez-Leiro R ACS Chem Biol. 2020 Jan 17;15(1):179-188. doi: 10.1021/acschembio.9b00752. Epub, 2019 Dec 24. PMID:31874027 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Galactosidase 3D structures

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