4c1t: Difference between revisions

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<StructureSection load='4c1t' size='340' side='right'caption='[[4c1t]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
<StructureSection load='4c1t' size='340' side='right'caption='[[4c1t]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4c1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Biflb Biflb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C1T FirstGlance]. <br>
<table><tr><td colspan='2'>[[4c1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_animalis_subsp._lactis_Bl-04 Bifidobacterium animalis subsp. lactis Bl-04]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C1T FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AHR:ALPHA-L-ARABINOFURANOSE'>AHR</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AHR:ALPHA-L-ARABINOFURANOSE'>AHR</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zkk|3zkk]], [[3zkl|3zkl]], [[4c1u|4c1u]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c1t OCA], [https://pdbe.org/4c1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c1t RCSB], [https://www.ebi.ac.uk/pdbsum/4c1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c1t ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c1t OCA], [http://pdbe.org/4c1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c1t RCSB], [http://www.ebi.ac.uk/pdbsum/4c1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c1t ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glycan utilization plays a key role in modulating the composition of the gut microbiota, but molecular insight into oligosaccharide uptake by this microbial community is lacking. Arabinoxylo-oligosaccharides (AXOS) are abundant in the diet, and are selectively fermented by probiotic bifidobacteria in the colon. Here we show how selectivity for AXOS uptake is established by the probiotic strain Bifidobacterium animalis subsp. lactis Bl-04. The binding protein BlAXBP, which is associated with an ATP-binding cassette (ABC) transporter that mediates the uptake of AXOS, displays an exceptionally broad specificity for arabinosyl-decorated and undecorated xylo-oligosaccharides, with preference for tri- and tetra-saccharides. Crystal structures of BlAXBP in complex with four different ligands revealed the basis for this versatility. Uniquely, the protein was able to recognize oligosaccharides in two opposite orientations, which facilitates the optimization of interactions with the various ligands. Broad substrate specificity was further enhanced by a spacious binding pocket accommodating decorations at different mainchain positions and conformational flexibility of a lid-like loop. Phylogenetic and genetic analyses show that BlAXBP is highly conserved within Bifidobacterium, but is lacking in other gut microbiota members. These data indicate niche adaptation within Bifidobacterium and highlight the metabolic syntrophy (cross-feeding) among the gut microbiota.
Structural basis for arabinoxylo-oligosaccharide capture by the probiotic Bifidobacterium animalis subsp. lactis Bl-04.,Ejby M, Fredslund F, Vujicic-Zagar A, Svensson B, Slotboom DJ, Abou Hachem M Mol Microbiol. 2013 Dec;90(5):1100-12. doi: 10.1111/mmi.12419. Epub 2013 Oct 31. PMID:24279727<ref>PMID:24279727</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4c1t" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Biflb]]
[[Category: Bifidobacterium animalis subsp. lactis Bl-04]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Ejby, M]]
[[Category: Abou Hachem M]]
[[Category: Fredslund, F]]
[[Category: Ejby M]]
[[Category: Hachem, M Abou]]
[[Category: Fredslund F]]
[[Category: Slotboom, D J]]
[[Category: Slotboom DJ]]
[[Category: Svensson, B]]
[[Category: Svensson B]]
[[Category: Vujicic-Zagar, A]]
[[Category: Vujicic-Zagar A]]
[[Category: Abc transport]]
[[Category: Prebiotic]]
[[Category: Probiotic]]
[[Category: Transport protein]]

Revision as of 17:21, 7 September 2022

Structure of the xylo-oligosaccharide specific solute binding protein from Bifidobacterium animalis subsp. lactis Bl-04 in complex with arabinoxylotriose

4c1t, resolution 2.39Å

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