1ag6: Difference between revisions

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[[Image:1ag6.jpg|left|200px]]
{{Seed}}
[[Image:1ag6.png|left|200px]]


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{{STRUCTURE_1ag6|  PDB=1ag6  |  SCENE=  }}  
{{STRUCTURE_1ag6|  PDB=1ag6  |  SCENE=  }}  


'''PLASTOCYANIN FROM SPINACH'''
===PLASTOCYANIN FROM SPINACH===




==Overview==
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The crystal structure of plastocyanin from spinach has been determined using molecular replacement, with the structure of plastocyanin from poplar as a search model. Successful crystallization was facilitated by site-directed mutagenesis in which residue Gly8 was substituted with Asp. The region around residue 8 was believed to be too mobile for the wild-type protein to form crystals despite extensive screening. The current structure represents the oxidized plastocyanin, copper (II), at low pH (approximately 4.4). In contrast to the similarity in the core region as compared to its poplar counterpart, the structure shows some significant differences in loop regions. The most notable is the large shift of the 59-61 loop where the largest shift is 3.0 A for the C(alpha) atom of Glu59. This results in different patterns of electrostatic potential around the acidic patches for the two proteins.
The line below this paragraph, {{ABSTRACT_PUBMED_9792096}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_9792096}}


==About this Structure==
==About this Structure==
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[[Category: Electron transfer]]
[[Category: Electron transfer]]
[[Category: Electron transport]]
[[Category: Electron transport]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:13:29 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:46:43 2008''

Revision as of 13:46, 30 June 2008

File:1ag6.png

Template:STRUCTURE 1ag6

PLASTOCYANIN FROM SPINACH

Template:ABSTRACT PUBMED 9792096

About this Structure

1AG6 is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.

Reference

Crystal structure of spinach plastocyanin at 1.7 A resolution., Xue Y, Okvist M, Hansson O, Young S, Protein Sci. 1998 Oct;7(10):2099-105. PMID:9792096

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