2ex5: Difference between revisions

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[[Image:2ex5.gif|left|200px]]<br />

[[Image:2ex5.gif|left|200px]]<br /><applet load="2ex5" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2ex5" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2ex5, resolution 2.200Å" />

'''Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA'''<br />

==Overview==

Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the, endonuclease ORF. We have determined the structure and DNA recognition, behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from, Chlamydomonas eugametos. This symmetric endonuclease displays unique, structural elaborations on its core enzyme fold, and it preferentially, cleaves a highly asymmetric target site. This latter property represents, an early step, prior to gene fusion, in the generation of asymmetric DNA, binding platforms from homodimeric ancestors. The divergence of the, sequence, structure, and target recognition behavior of homing, endonucleases, as illustrated by this study, leads to the invasion of, novel genomic sites by mobile introns during evolution.

Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.

==About this Structure==

2EX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_eugametos Chlamydomonas eugametos] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA].

2EX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_eugametos Chlamydomonas eugametos] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA].

==Reference==

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[[Category: Chlamydomonas eugametos]]

[[Category: Single protein]]

[[Category: Spiegel, P.C.]]

[[Category: Spiegel, P C.]]

[[Category: Stoddard, B.L.]]

[[Category: Stoddard, B L.]]

[[Category: CA]]

[[Category: homing endonuclease]]

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[[Category: protein/dna complex]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:15:29 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2ex5.gif|left|200px]]<br />
+[[Image:2ex5.gif|left|200px]]<br /><applet load="2ex5" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2ex5" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2ex5, resolution 2.200&Aring;" />
 '''Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA'''<br />
 ==Overview==
-Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the, endonuclease ORF. We have determined the structure and DNA recognition, behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from, Chlamydomonas eugametos. This symmetric endonuclease displays unique, structural elaborations on its core enzyme fold, and it preferentially, cleaves a highly asymmetric target site. This latter property represents, an early step, prior to gene fusion, in the generation of asymmetric DNA, binding platforms from homodimeric ancestors. The divergence of the, sequence, structure, and target recognition behavior of homing, endonucleases, as illustrated by this study, leads to the invasion of, novel genomic sites by mobile introns during evolution.
+Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.
 ==About this Structure==
-EX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_eugametos Chlamydomonas eugametos] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA].
+EX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_eugametos Chlamydomonas eugametos] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Chlamydomonas eugametos]]
 [[Category: Single protein]]
-[[Category: Spiegel, P.C.]]
+[[Category: Spiegel, P C.]]
-[[Category: Stoddard, B.L.]]
+[[Category: Stoddard, B L.]]
 [[Category: CA]]
 [[Category: homing endonuclease]]
@@ Line 22: / Line 21: @@
 [[Category: protein/dna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 12:47:16 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:15:29 2008''