6vhc

1.4A damaged structure of GSNQNNF used to determine initial phases from radiation damage

spinqualitylabelsall models 6vhc, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6vhc is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT, ZN Related: 6clj Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to determine atomic-resolution structures from vanishingly small three-dimensional crystals. Here, we present an example of an experimentally phased structure using only MicroED data. The structure of a seven-residue peptide is solved starting from differences to the diffraction intensities induced by structural changes due to radiation damage. The same wedge of reciprocal space was recorded twice by continuous-rotation MicroED from a set of 11 individual crystals. The data from the first pass were merged to make a "low-dose dataset." The data from the second pass were similarly merged to form a "damaged dataset." Differences between these two datasets were used to identify a single heavy-atom site from a Patterson difference map, and initial phases were generated. Finally, the structure was completed by iterative cycles of modeling and refinement. Experimental Phasing of MicroED Data Using Radiation Damage.,Martynowycz MW, Hattne J, Gonen T Structure. 2020 Feb 4. pii: S0969-2126(20)30008-3. doi:, 10.1016/j.str.2020.01.008. PMID:32023481 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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