1buw: Difference between revisions
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New page: left|200px<br /> <applet load="1buw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1buw, resolution 1.9Å" /> '''CRYSTAL STRUCTURE OF... |
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==Overview== | ==Overview== | ||
Although numerous reports have documented that the S-nitrosylation of, cysteine residues by NO alters the activities of a wide variety of, proteins, the direct visualization and the structural consequences of this, reversible modification have not yet been reported for any protein. Here, we describe the crystal structure of S-nitroso-nitrosylhemoglobin, determined at a resolution of 1.8 A. The specific reaction of NO with, Cys93beta is confirmed in this structure, and a large, S-nitrosylation-induced change in the tertiary structure of the, COOH-terminal dipeptides of the beta subunits provides additional insight, into the stereochemical mechanism by which blood flow is regulated by the, interaction of NO with hemoglobin. | Although numerous reports have documented that the S-nitrosylation of, cysteine residues by NO alters the activities of a wide variety of, proteins, the direct visualization and the structural consequences of this, reversible modification have not yet been reported for any protein. Here, we describe the crystal structure of S-nitroso-nitrosylhemoglobin, determined at a resolution of 1.8 A. The specific reaction of NO with, Cys93beta is confirmed in this structure, and a large, S-nitrosylation-induced change in the tertiary structure of the, COOH-terminal dipeptides of the beta subunits provides additional insight, into the stereochemical mechanism by which blood flow is regulated by the, interaction of NO with hemoglobin. | ||
==Disease== | |||
Known diseases associated with this structure: Erythremias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Erythremias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Erythrocytosis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], HPFH, deletion type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Heinz body anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Heinz body anemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Heinz body anemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Hemoglobin H disease OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Hypochromic microcytic anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Methemoglobinemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Methemoglobinemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Sickle cell anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Thalassemia, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Thalassemia-beta, dominant inclusion-body OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Thalassemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Thalassemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]] | |||
==About this Structure== | ==About this Structure== | ||
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[[Category: oxygen transport and vasodilation]] | [[Category: oxygen transport and vasodilation]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:14:45 2007'' | ||
