1atp: Difference between revisions

Line 1:

[[Image:1atp.~~gif~~|left|200px]]

[[Image:1atp.png|left|200px]]

<!--

Line 9:

Line 10:

~~'''~~2.2 ANGSTROM REFINED CRYSTAL STRUCTURE OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE COMPLEXED WITH MNATP AND A PEPTIDE INHIBITOR~~'''~~

===2.2 ANGSTROM REFINED CRYSTAL STRUCTURE OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE COMPLEXED WITH MNATP AND A PEPTIDE INHIBITOR===

~~==Overview==~~

<!--

. The ~~crystal structure of a ternary complex containing the catalytic subunit of cAMP-dependent protein kinase~~, ~~ATP and a 20-residue inhibitor peptide was refined at a resolution of 2.2 A to an R value of 0.177. In order to identify the metal binding sites~~, the ~~crystals, originally grown in~~ the ~~presence of low concentrations of Mg~~(~~2+), were soaked in Mn(2+)~~. ~~Two Mn(2+) ions were identified using an anomalous Fourier map~~. ~~One Mn(2+~~) ~~ion bridges~~ the ~~gamma~~- ~~and beta~~-phosphates and interacts with Asp184 and two water molecules. The second Mn(2+) ion interacts with the side chains of Asn171 and Asp l84 as well as with a water molecule. Modeling a serine into the P site of the inhibitor peptide suggests a mechanism for phosphotransfer.

The line below this paragraph, {{ABSTRACT_PUBMED_15299527}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15299527 is the PubMed ID number.

-->

==About this Structure==

Line 30:

Line 34:

[[Category: Xuong, N H.]]

[[Category: Zheng, J.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May 2 10~~:40:45 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:34:32 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1atp.gif|left|200px]]
+{{Seed}}
+[[Image:1atp.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_1atp|  PDB=1atp  |  SCENE=  }}
-'''2.2 ANGSTROM REFINED CRYSTAL STRUCTURE OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE COMPLEXED WITH MNATP AND A PEPTIDE INHIBITOR'''
+===2.2 ANGSTROM REFINED CRYSTAL STRUCTURE OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE COMPLEXED WITH MNATP AND A PEPTIDE INHIBITOR===
-==Overview==
+<!--
-. The crystal structure of a ternary complex containing the catalytic subunit of cAMP-dependent protein kinase, ATP and a 20-residue inhibitor peptide was refined at a resolution of 2.2 A to an R value of 0.177. In order to identify the metal binding sites, the crystals, originally grown in the presence of low concentrations of Mg(2+), were soaked in Mn(2+). Two Mn(2+) ions were identified using an anomalous Fourier map. One Mn(2+) ion bridges the gamma- and beta-phosphates and interacts with Asp184 and two water molecules. The second Mn(2+) ion interacts with the side chains of Asn171 and Asp l84 as well as with a water molecule. Modeling a serine into the P site of the inhibitor peptide suggests a mechanism for phosphotransfer.
+The line below this paragraph, {{ABSTRACT_PUBMED_15299527}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15299527 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15299527}}
 ==About this Structure==
@@ Line 30: / Line 34: @@
 [[Category: Xuong, N H.]]
 [[Category: Zheng, J.]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:40:45 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:34:32 2008''