6kvo: Difference between revisions

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<StructureSection load='6kvo' size='340' side='right'caption='[[6kvo]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='6kvo' size='340' side='right'caption='[[6kvo]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6kvo]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KVO FirstGlance]. <br>
<table><tr><td colspan='2'>[[6kvo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KVO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvo OCA], [http://pdbe.org/6kvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kvo RCSB], [http://www.ebi.ac.uk/pdbsum/6kvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvo ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvo OCA], [https://pdbe.org/6kvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kvo RCSB], [https://www.ebi.ac.uk/pdbsum/6kvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvo ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A1S4CVP6_TOBAC A0A1S4CVP6_TOBAC]
Holliday junctions (HJs) are key DNA intermediates in genetic recombination and are eliminated by nuclease, termed resolvase, to ensure genome stability. HJ resolvases have been identified across all kingdoms of life, members of which exhibit sequence-dependent HJ resolution. However, the molecular basis of sequence selectivity remains largely unknown. Here, we present the chloroplast resolvase MOC1, which cleaves HJ in a cytosine-dependent manner. We determine the crystal structure of MOC1 with and without HJs. MOC1 exhibits an RNase H fold, belonging to the retroviral integrase family. MOC1 functions as a dimer, and the HJ is embedded into the basic cleft of the dimeric enzyme. We characterize a base recognition loop (BR loop) that protrudes into and opens the junction. Residues from the BR loop intercalate into the bases, disrupt the C-G base pairing at the crossover and recognize the cytosine, providing the molecular basis for sequence-dependent HJ resolution by a resolvase.


Structural insights into sequence-dependent Holliday junction resolution by the chloroplast resolvase MOC1.,Yan J, Hong S, Guan Z, He W, Zhang D, Yin P Nat Commun. 2020 Mar 17;11(1):1417. doi: 10.1038/s41467-020-15242-8. PMID:32184398<ref>PMID:32184398</ref>
==See Also==
 
*[[Resolvase 3D structures|Resolvase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6kvo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Guan, Z Y]]
[[Category: Nicotiana tabacum]]
[[Category: Hong, S X]]
[[Category: Synthetic construct]]
[[Category: Yan, J J]]
[[Category: Guan ZY]]
[[Category: Yin, P]]
[[Category: Hong SX]]
[[Category: Chloroplast]]
[[Category: Yan JJ]]
[[Category: Holliday junction]]
[[Category: Yin P]]
[[Category: Plant protein]]
[[Category: Plant protein-dna complex]]
[[Category: Resolvase]]

Latest revision as of 10:44, 27 March 2024

Crystal structure of chloroplast resolvase in complex with Holliday junction

6kvo, resolution 2.50Å

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