6t73: Difference between revisions

Latest revision as of 12:56, 24 January 2024

New antiparallel dimer of aureochrome 1a LOV domain mutants from Phaeodactylum tricornutum

Structural highlights

6t73 is a 3 chain structure with sequence from Phaeodactylum tricornutum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.435Å
Ligands:	CL, FMN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A140UHJ0_PHATR

Publication Abstract from PubMed

Control of cellular events by optogenetic tools is a powerful approach to manipulate cellular functions in a minimally invasive manner. A common problem posed by the application of optogenetic tools is to tune the activity range to be physiologically relevant. Here, we characterized a photoreceptor of the light-oxygen-voltage domain family of Phaeodactylum tricornutum aureochrome 1a (AuLOV) as a tool for increasing protein stability under blue light conditions in budding yeast. Structural studies of AuLOV(wt), the variants AuLOV(M254) and AuLOV(W349) revealed alternative dimer association modes for the dark state, which differ from previously reported AuLOV dark state structures. Rational design of AuLOV-dimer interface mutations resulted in an optimized optogenetic tool that we fused to the photoactivatable adenylyl cyclase from Beggiatoa sp.. This synergistic light-regulation approach using two photoreceptors resulted in an optimized, photoactivatable adenylyl cyclase with a cyclic AMP production activity that matches the physiological range of Saccharomyces cerevisiae. Overall, we enlarged the optogenetic toolbox for yeast and demonstrated the importance of fine-tuning the optogenetic tool activity for successful application in cells.

An optogenetic tool for induced protein stabilization based on the Phaeodactylum tricornutum aureochrome 1a LOV domain.,Hepp S, Trauth J, Hasenjager S, Bezold F, Essen LO, Taxis C J Mol Biol. 2020 Feb 24. pii: S0022-2836(20)30192-3. doi:, 10.1016/j.jmb.2020.02.019. PMID:32105734^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hepp S, Trauth J, Hasenjager S, Bezold F, Essen LO, Taxis C. An optogenetic tool for induced protein stabilization based on the Phaeodactylum tricornutum aureochrome 1a LOV domain. J Mol Biol. 2020 Feb 24. pii: S0022-2836(20)30192-3. doi:, 10.1016/j.jmb.2020.02.019. PMID:32105734 doi:https://dx.doi.org/10.1016/j.jmb.2020.02.019

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OCA

[1] Hepp S, Trauth J, Hasenjager S, Bezold F, Essen LO, Taxis C. An optogenetic tool for induced protein stabilization based on the Phaeodactylum tricornutum aureochrome 1a LOV domain. J Mol Biol. 2020 Feb 24. pii: S0022-2836(20)30192-3. doi:, 10.1016/j.jmb.2020.02.019. PMID:32105734 doi:https://dx.doi.org/10.1016/j.jmb.2020.02.019

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='6t73' size='340' side='right'caption='[[6t73]], [[Resolution|resolution]] 3.44&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6t73]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Diatom Diatom]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T73 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6T73 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6t73]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaeodactylum_tricornutum Phaeodactylum tricornutum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T73 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.435&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6t73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t73 OCA], [http://pdbe.org/6t73 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t73 RCSB], [http://www.ebi.ac.uk/pdbsum/6t73 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t73 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t73 OCA], [https://pdbe.org/6t73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t73 RCSB], [https://www.ebi.ac.uk/pdbsum/6t73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t73 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A140UHJ0_PHATR A0A140UHJ0_PHATR]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Diatom]]
 [[Category: Large Structures]]
-[[Category: Essen, L O]]
+[[Category: Phaeodactylum tricornutum]]
-[[Category: Hepp, S]]
+[[Category: Essen LO]]
-[[Category: Flavoprotein]]
+[[Category: Hepp S]]
-[[Category: Interface]]
-[[Category: Photoreceptor]]

6t73: Difference between revisions

Latest revision as of 12:56, 24 January 2024

New antiparallel dimer of aureochrome 1a LOV domain mutants from Phaeodactylum tricornutum

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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