5lkh

Cryo-EM structure of the Tc toxin TcdA1 in its pore state (obtained by flexible fitting)

Loading... Status: waiting for Mol* initialization Structural highlights 5lkh is a 5 chain structure with sequence from Photorhabdus luminescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.46Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9RN43_PHOLU Publication Abstract from PubMed Tc toxins from pathogenic bacteria use a special syringe-like mechanism to perforate the host cell membrane and inject a deadly enzyme into the host cytosol. The molecular mechanism of this unusual injection system is poorly understood. Using electron cryomicroscopy, we determined the structure of TcdA1 from Photorhabdus luminescens embedded in lipid nanodiscs. In our structure, compared with the previous structure of TcdA1 in the prepore state, the transmembrane helices rearrange in the membrane and open the initially closed pore. However, the helices do not span the complete membrane; instead, the loops connecting the helices form the rim of the funnel. Lipid head groups reach into the space between the loops and consequently stabilize the pore conformation. The linker domain is folded and packed into a pocket formed by the other domains of the toxin, thereby considerably contributing to stabilization of the pore state. Membrane insertion of a Tc toxin in near-atomic detail.,Gatsogiannis C, Merino F, Prumbaum D, Roderer D, Leidreiter F, Meusch D, Raunser S Nat Struct Mol Biol. 2016 Aug 29. doi: 10.1038/nsmb.3281. PMID:27571177 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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