5n8n: Difference between revisions

Latest revision as of 12:35, 15 November 2023

Contracted sheath of a Pseudomonas aeruginosa type six secretion system consisting of TssB1 and TssC1

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Structural highlights

5n8n is a 30 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.28Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TSSB1_PSEAE Core component of the H1 type VI (H1-T6SS) secretion system that plays a role in the release of toxins targeting both eukaryotic and prokaryotic species. Forms the sheath of the structure by assembling into tubules together with TssC1 resulting in the stacking of cogwheel-like structures showing predominantly a 12-fold symmetry (PubMed:23341461, PubMed:25305017, PubMed:22906320). The sheath contracts to provide the energy needed for effector delivery (PubMed:29307484).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Pseudomonas aeruginosa has three type VI secretion systems (T6SSs), H1-, H2-, and H3-T6SS, each belonging to a distinct group. The two T6SS components, TssB/VipA and TssC/VipB, assemble to form tubules that conserve structural/functional homology with tail sheaths of contractile bacteriophages and pyocins. Here, we used cryoelectron microscopy to solve the structure of the H1-T6SS P. aeruginosa TssB1C1 sheath at 3.3 A resolution. Our structure allowed us to resolve some features of the T6SS sheath that were not resolved in the Vibrio cholerae VipAB and Francisella tularensis IglAB structures. Comparison with sheath structures from other contractile machines, including T4 phage and R-type pyocins, provides a better understanding of how these systems have conserved similar functions/mechanisms despite evolution. We used the P. aeruginosa R2 pyocin as a structural template to build an atomic model of the TssB1C1 sheath in its extended conformation, allowing us to propose a coiled-spring-like mechanism for T6SS sheath contraction.

Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa.,Salih O, He S, Planamente S, Stach L, MacDonald JT, Manoli E, Scheres SHW, Filloux A, Freemont PS Structure. 2017 Dec 21. pii: S0969-2126(17)30404-5. doi:, 10.1016/j.str.2017.12.005. PMID:29307484^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lossi NS, Manoli E, Simpson P, Jones C, Hui K, Dajani R, Coulthurst SJ, Freemont P, Filloux A. The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system. Mol Microbiol. 2012 Oct;86(2):437-56. PMID:22906320 doi:10.1111/j.1365-2958.2012.08204.x
↑ Lossi NS, Manoli E, Förster A, Dajani R, Pape T, Freemont P, Filloux A. The HsiB1C1 (TssB-TssC) complex of the Pseudomonas aeruginosa type VI secretion system forms a bacteriophage tail sheathlike structure. J Biol Chem. 2013 Mar 15;288(11):7536-7548. PMID:23341461 doi:10.1074/jbc.M112.439273
↑ Forster A, Planamente S, Manoli E, Lossi NS, Freemont PS, Filloux A. Coevolution of the ATPase ClpV, the Sheath Proteins TssB and TssC and the Accessory Protein TagJ/HsiE1 Distinguishes Type VI Secretion Classes. J Biol Chem. 2014 Oct 10. pii: jbc.M114.600510. PMID:25305017 doi:https://dx.doi.org/10.1074/jbc.M114.600510
↑ Salih O, He S, Planamente S, Stach L, MacDonald JT, Manoli E, Scheres SHW, Filloux A, Freemont PS. Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa. Structure. 2017 Dec 21. pii: S0969-2126(17)30404-5. doi:, 10.1016/j.str.2017.12.005. PMID:29307484 doi:https://dx.doi.org/10.1016/j.str.2017.12.005
↑ Salih O, He S, Planamente S, Stach L, MacDonald JT, Manoli E, Scheres SHW, Filloux A, Freemont PS. Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa. Structure. 2017 Dec 21. pii: S0969-2126(17)30404-5. doi:, 10.1016/j.str.2017.12.005. PMID:29307484 doi:https://dx.doi.org/10.1016/j.str.2017.12.005

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Lossi NS, Manoli E, Simpson P, Jones C, Hui K, Dajani R, Coulthurst SJ, Freemont P, Filloux A. The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system. Mol Microbiol. 2012 Oct;86(2):437-56. PMID:22906320 doi:10.1111/j.1365-2958.2012.08204.x

[2] Lossi NS, Manoli E, Förster A, Dajani R, Pape T, Freemont P, Filloux A. The HsiB1C1 (TssB-TssC) complex of the Pseudomonas aeruginosa type VI secretion system forms a bacteriophage tail sheathlike structure. J Biol Chem. 2013 Mar 15;288(11):7536-7548. PMID:23341461 doi:10.1074/jbc.M112.439273

[3] Forster A, Planamente S, Manoli E, Lossi NS, Freemont PS, Filloux A. Coevolution of the ATPase ClpV, the Sheath Proteins TssB and TssC and the Accessory Protein TagJ/HsiE1 Distinguishes Type VI Secretion Classes. J Biol Chem. 2014 Oct 10. pii: jbc.M114.600510. PMID:25305017 doi:https://dx.doi.org/10.1074/jbc.M114.600510

[4] Salih O, He S, Planamente S, Stach L, MacDonald JT, Manoli E, Scheres SHW, Filloux A, Freemont PS. Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa. Structure. 2017 Dec 21. pii: S0969-2126(17)30404-5. doi:, 10.1016/j.str.2017.12.005. PMID:29307484 doi:https://dx.doi.org/10.1016/j.str.2017.12.005

[5] Salih O, He S, Planamente S, Stach L, MacDonald JT, Manoli E, Scheres SHW, Filloux A, Freemont PS. Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa. Structure. 2017 Dec 21. pii: S0969-2126(17)30404-5. doi:, 10.1016/j.str.2017.12.005. PMID:29307484 doi:https://dx.doi.org/10.1016/j.str.2017.12.005

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@@ Line 3: / Line 3: @@
 <SX load='5n8n' size='340' side='right' viewer='molstar' caption='[[5n8n]], [[Resolution|resolution]] 3.28&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5n8n]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N8N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N8N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5n8n]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N8N FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AO964_32215, PAERUG_E15_London_28_01_14_04448, PAERUG_P32_London_17_VIM_2_10_11_02574, PAMH19_0082 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885]), U769_00445 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.28&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8n OCA], [http://pdbe.org/5n8n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n8n RCSB], [http://www.ebi.ac.uk/pdbsum/5n8n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8n ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8n OCA], [https://pdbe.org/5n8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n8n RCSB], [https://www.ebi.ac.uk/pdbsum/5n8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8n ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/TSSB1_PSEAE TSSB1_PSEAE] Core component of the H1 type VI (H1-T6SS) secretion system that plays a role in the release of toxins targeting both eukaryotic and prokaryotic species. Forms the sheath of the structure by assembling into tubules together with TssC1 resulting in the stacking of cogwheel-like structures showing predominantly a 12-fold symmetry (PubMed:23341461, PubMed:25305017, PubMed:22906320). The sheath contracts to provide the energy needed for effector delivery (PubMed:29307484).<ref>PMID:22906320</ref> <ref>PMID:23341461</ref> <ref>PMID:25305017</ref> <ref>PMID:29307484</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 </SX>
 [[Category: Large Structures]]
-[[Category: Filloux, A]]
+[[Category: Pseudomonas aeruginosa]]
-[[Category: Freemont, P S]]
+[[Category: Filloux A]]
-[[Category: He, S]]
+[[Category: Freemont PS]]
-[[Category: Macdonald, J T]]
+[[Category: He S]]
-[[Category: Manoli, E]]
+[[Category: Macdonald JT]]
-[[Category: Planamente, S]]
+[[Category: Manoli E]]
-[[Category: Salih, O]]
+[[Category: Planamente S]]
-[[Category: Scheres, S]]
+[[Category: Salih O]]
-[[Category: Stach, L]]
+[[Category: Scheres S]]
-[[Category: Structural protein]]
+[[Category: Stach L]]
-[[Category: Type six secretion system]]

5n8n: Difference between revisions

Latest revision as of 12:35, 15 November 2023

Contracted sheath of a Pseudomonas aeruginosa type six secretion system consisting of TssB1 and TssC1

Structural highlights

Function

Publication Abstract from PubMed

References

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