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<SX load='6njp' size='340' side='right' viewer='molstar' caption='[[6njp]], [[Resolution|resolution]] 3.29&Aring;' scene=''>
<SX load='6njp' size='340' side='right' viewer='molstar' caption='[[6njp]], [[Resolution|resolution]] 3.29&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6njp]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco27 Eco27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NJP FirstGlance]. <br>
<table><tr><td colspan='2'>[[6njp]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O127:H6_str._E2348/69 Escherichia coli O127:H6 str. E2348/69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NJP FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.29&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">escN, E2348C_3948 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=574521 ECO27]), E2348C_3947 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=574521 ECO27])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6njp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6njp OCA], [http://pdbe.org/6njp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6njp RCSB], [http://www.ebi.ac.uk/pdbsum/6njp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6njp ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6njp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6njp OCA], [https://pdbe.org/6njp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6njp RCSB], [https://www.ebi.ac.uk/pdbsum/6njp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6njp ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/B7UMA6_ECO27 B7UMA6_ECO27]
Many Gram-negative bacteria, including causative agents of dysentery, plague, and typhoid fever, rely on a type III secretion system - a multi-membrane spanning syringe-like apparatus - for their pathogenicity. The cytosolic ATPase complex of this injectisome is proposed to play an important role in energizing secretion events and substrate recognition. We present the 3.3 A resolution cryo-EM structure of the enteropathogenic Escherichia coli ATPase EscN in complex with its central stalk EscO. The structure shows an asymmetric pore with different functional states captured in its six catalytic sites, details directly supporting a rotary catalytic mechanism analogous to that of the heterohexameric F1/V1-ATPases despite its homohexameric nature. Situated at the C-terminal opening of the EscN pore is one molecule of EscO, with primary interaction mediated through an electrostatic interface. The EscN-EscO structure provides significant atomic insights into how the ATPase contributes to type III secretion, including torque generation and binding of chaperone/substrate complexes.
 
Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry.,Majewski DD, Worrall LJ, Hong C, Atkinson CE, Vuckovic M, Watanabe N, Yu Z, Strynadka NCJ Nat Commun. 2019 Feb 7;10(1):626. doi: 10.1038/s41467-019-08477-7. PMID:30733444<ref>PMID:30733444</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6njp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Eco27]]
[[Category: Escherichia coli O127:H6 str. E2348/69]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Atkinson, C E]]
[[Category: Atkinson CE]]
[[Category: Hong, C]]
[[Category: Hong C]]
[[Category: Majewski, D D]]
[[Category: Majewski DD]]
[[Category: Strynadka, N C.J]]
[[Category: Strynadka NCJ]]
[[Category: Vuckovic, M]]
[[Category: Vuckovic M]]
[[Category: Watanabe, N]]
[[Category: Watanabe N]]
[[Category: Worrall, L J]]
[[Category: Worrall LJ]]
[[Category: Yu, Z]]
[[Category: Yu Z]]
[[Category: Adp]]
[[Category: Atpase]]
[[Category: Hexamer]]
[[Category: Hydrolase]]
[[Category: Type iii secretion system]]

Revision as of 09:21, 20 March 2024

Structure of the assembled ATPase EscN in complex with its central stalk EscO from the enteropathogenic E. coli (EPEC) type III secretion system

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