1b3c: Difference between revisions

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[[Image:1b3c.gif|left|200px]]
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[[Image:1b3c.png|left|200px]]


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{{STRUCTURE_1b3c|  PDB=1b3c  |  SCENE=  }}  
{{STRUCTURE_1b3c|  PDB=1b3c  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF A BETA-NEUROTOXIN FROM THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING'''
===SOLUTION STRUCTURE OF A BETA-NEUROTOXIN FROM THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING===




==Overview==
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We report the detailed solution structure of the 7.2 kDa protein CsE-I, a beta-neurotoxin from the New World scorpion Centruroides sculpturatus Ewing. This toxin binds to sodium channels, but unlike the alpha-neurotoxins, shifts the voltage of activation toward more negative potentials causing the membrane to fire spontaneously. Sequence-specific proton NMR assignments were made using 600 MHz 2D-NMR data. Distance geometry and dynamical simulated annealing refinements were performed using experimental distance and torsion angle constraints from NOESY and pH-COSY data. A family of 40 structures without constraint violations was generated, and an energy-minimized average structure was computed. The backbone conformation of the CsE-I toxin shows similar secondary structural features as the prototypical alpha-neurotoxin, CsE-v3, and is characterized by a short 2(1/2)-turn alpha-helix and a 3-strand antiparallel beta-sheet, both held together by disulfide bridges. The RMSD for the backbone atoms between CsE-I and CsE-v3 is 1.48 A. Despite this similarity in the overall backbone folding, the these two proteins show some important differences in the primary structure (sequence) and electrostatic potential surfaces. Our studies provide a basis for unravelling the role of these differences in relation to the known differences in the receptor sites on the voltage sensitive sodium channel for the alpha- and beta-neurotoxins.
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{{ABSTRACT_PUBMED_9918851}}


==About this Structure==
==About this Structure==
1B3C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3C OCA].  
1B3C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3C OCA].  


==Reference==
==Reference==
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[[Category: New world toxin]]
[[Category: New world toxin]]
[[Category: Scorpion neurotoxin]]
[[Category: Scorpion neurotoxin]]
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Revision as of 15:08, 30 June 2008

File:1b3c.png

Template:STRUCTURE 1b3c

SOLUTION STRUCTURE OF A BETA-NEUROTOXIN FROM THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING

Template:ABSTRACT PUBMED 9918851

About this Structure

1B3C is a Single protein structure of sequence from Centruroides sculpturatus. Full experimental information is available from OCA.

Reference

Solution structure of a beta-neurotoxin from the New World scorpion Centruroides sculpturatus Ewing., Jablonsky MJ, Jackson PL, Trent JO, Watt DD, Krishna NR, Biochem Biophys Res Commun. 1999 Jan 19;254(2):406-12. PMID:9918851

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