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| [[Image:1b3p.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1b3p| PDB=1b3p | SCENE= }} | | {{STRUCTURE_1b3p| PDB=1b3p | SCENE= }} |
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| '''5'-D(*GP*GP*AP*GP*GP*AP*T)-3''''
| | ===5'-D(*GP*GP*AP*GP*GP*AP*T)-3'=== |
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| ==Overview==
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| BACKGROUND: Triplet repeat sequences are of considerable biological importance as the expansion of such tandem arrays can lead to the onset of a range of human diseases. Such sequences can self-pair via mismatch alignments to form higher order structures that have the potential to cause replication blocks, followed by strand slippage and sequence expansion. The all-purine d(GGA)n triplet repeat sequence is of particular interest because purines can align via G.G, A.A and G.A mismatch formation. RESULTS: We have solved the structure of the uniformly 13C,15N-labeled d(G1-G2-A3-G4-G5-A6-T7) sequence in 10 mM Na+ solution. This sequence adopts a novel twofold-symmetric duplex fold where interlocked V-shaped arrowhead motifs are aligned solely via interstrand G1.G4, G2.G5 and A3.A6 mismatch formation. The tip of the arrowhead motif is centered about the p-A3-p step, and symmetry-related local parallel-stranded duplex domains are formed by the G1-G2-A3 and G4-G5-A6 segments of partner strands. CONCLUSIONS: The purine-rich (GGA)n triplet repeat sequence is dispersed throughout the eukaryotic genome. Several features of the arrowhead duplex motif for the (GGA)2 triplet repeat provide a unique scaffold for molecular recognition. These include the large localized bend in the sugar-phosphate backbones, the segmental parallel-stranded alignment of strands and the exposure of the Watson-Crick edges of several mismatched bases.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10425682}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10425682 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10425682}} |
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| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3P OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3P OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Uniform 13c]] | | [[Category: Uniform 13c]] |
| [[Category: V-shaped backbone]] | | [[Category: V-shaped backbone]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:01:29 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:10:25 2008'' |
