6ood: Difference between revisions
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==Structure of the pterocarpan synthase dirigent protein PsPTS1== | ==Structure of the pterocarpan synthase dirigent protein PsPTS1== | ||
<StructureSection load='6ood' size='340' side='right'caption='[[6ood]]' scene=''> | <StructureSection load='6ood' size='340' side='right'caption='[[6ood]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OOD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OOD FirstGlance]. <br> | <table><tr><td colspan='2'>[[6ood]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Garden_pea Garden pea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OOD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OOD FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ood FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ood OCA], [http://pdbe.org/6ood PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ood RCSB], [http://www.ebi.ac.uk/pdbsum/6ood PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ood ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ood FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ood OCA], [http://pdbe.org/6ood PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ood RCSB], [http://www.ebi.ac.uk/pdbsum/6ood PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ood ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The biochemical activities of dirigent proteins (DPs) give rise to distinct complex classes of plant phenolics. DPs apparently began to emerge during the aquatic-to-land transition, with phylogenetic analyses revealing the presence of numerous DP subfamilies in the plant kingdom. The vast majority (>95%) of DPs in these large multigene families still await discovery of their biochemical functions. Here, we elucidated the 3D structures of two pterocarpan-forming proteins with dirigent-like domains. Both proteins stereospecifically convert distinct diastereomeric chiral isoflavonoid precursors to the chiral pterocarpans, (-)- and (+)-medicarpin, respectively. Their 3D structures enabled comparisons with stereoselective lignan- and aromatic terpenoid-forming DP orthologs. Each protein provides entry into diverse plant natural products classes, and our experiments suggest a common biochemical mechanism in binding and stabilizing distinct plant phenol-derived mono- and bis-quinone methide intermediates during different C-C and C-O bond-forming processes. These observations provide key insights into both their appearance and functional diversification of DPs during land plant evolution/adaptation. The proposed biochemical mechanisms based on our findings provide important clues to how additional physiological roles for DPs and proteins harboring dirigent-like domains can now be rationally and systematically identified. | |||
Pterocarpan synthase (PTS) structures suggest a common quinone methide-stabilizing function in dirigent proteins and proteins with dirigent-like domains.,Meng Q, Moinuddin SGA, Kim SJ, Bedgar DL, Costa MA, Thomas DG, Young RP, Smith CA, Cort JR, Davin LB, Lewis NG J Biol Chem. 2020 Aug 14;295(33):11584-11601. doi: 10.1074/jbc.RA120.012444. Epub, 2020 Jun 21. PMID:32565424<ref>PMID:32565424</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6ood" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Garden pea]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Smith | [[Category: Smith, C A]] | ||
[[Category: Apo-form]] | |||
[[Category: Dirigent protein]] | |||
[[Category: Plant protein]] | |||
Revision as of 08:07, 11 November 2020
Structure of the pterocarpan synthase dirigent protein PsPTS1
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