5f7q: Difference between revisions

Latest revision as of 06:41, 19 July 2023

ROK repressor Lmo0178 from Listeria monocytogenes bound to operator

Structural highlights

5f7q is a 6 chain structure with sequence from Listeria monocytogenes EGD-e. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.4Å
Ligands:	7PE, PEG, PG4, PG5, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8YAF1_LISMO

Publication Abstract from PubMed

Here we employ a 'systems structural biology' approach to functionally characterize an unconventional alpha-glucan metabolic pathway from the food-borne pathogen Listeria monocytogenes (Lm). Crystal structure determination coupled with basic biochemical and biophysical assays allowed for the identification of anabolic, transport, catabolic and regulatory portions of the cycloalternan pathway. These findings provide numerous insights into cycloalternan pathway function and reveal the mechanism of repressor, open reading frame, kinase (ROK) transcription regulators. Moreover, by developing a structural overview we were able to anticipate the cycloalternan pathway's role in the metabolism of partially hydrolysed starch derivatives and demonstrate its involvement in Lm pathogenesis. These findings suggest that the cycloalternan pathway plays a role in interspecies resource competition-potentially within the host gastrointestinal tract-and establish the methodological framework for characterizing bacterial systems of unknown function.

Structure to function of an alpha-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis.,Light SH, Cahoon LA, Halavaty AS, Freitag NE, Anderson WF Nat Microbiol. 2016 Nov 7;2:16202. doi: 10.1038/nmicrobiol.2016.202. PMID:27819654^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Light SH, Cahoon LA, Halavaty AS, Freitag NE, Anderson WF. Structure to function of an alpha-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis. Nat Microbiol. 2016 Nov 7;2:16202. doi: 10.1038/nmicrobiol.2016.202. PMID:27819654 doi:https://dx.doi.org/10.1038/nmicrobiol.2016.202

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OCA

[1] Light SH, Cahoon LA, Halavaty AS, Freitag NE, Anderson WF. Structure to function of an alpha-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis. Nat Microbiol. 2016 Nov 7;2:16202. doi: 10.1038/nmicrobiol.2016.202. PMID:27819654 doi:https://dx.doi.org/10.1038/nmicrobiol.2016.202

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='5f7q' size='340' side='right'caption='[[5f7q]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5f7q]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lismo Lismo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F7Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5F7Q FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5f7q]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F7Q FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7PE:2-(2-(2-(2-(2-(2-ETHOXYETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHANOL'>7PE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f7r|5f7r]], [[5f7v|5f7v]], [[5f7p|5f7p]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7PE:2-(2-(2-(2-(2-(2-ETHOXYETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHANOL'>7PE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lmo0178 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169963 LISMO])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f7q OCA], [https://pdbe.org/5f7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f7q RCSB], [https://www.ebi.ac.uk/pdbsum/5f7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f7q ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5f7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f7q OCA], [http://pdbe.org/5f7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f7q RCSB], [http://www.ebi.ac.uk/pdbsum/5f7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f7q ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8YAF1_LISMO Q8YAF1_LISMO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lismo]]
+[[Category: Listeria monocytogenes EGD-e]]
-[[Category: Anderson, W F]]
+[[Category: Anderson WF]]
-[[Category: Structural genomic]]
+[[Category: Light SH]]
-[[Category: Light, S H]]
-[[Category: Csgid]]
-[[Category: Kinase]]
-[[Category: Open reading frame]]
-[[Category: Repressor]]
-[[Category: Transcription-dna complex]]

5f7q: Difference between revisions

Latest revision as of 06:41, 19 July 2023

ROK repressor Lmo0178 from Listeria monocytogenes bound to operator

Structural highlights

Function

Publication Abstract from PubMed

References

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