6kxf: Difference between revisions
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==The ishigamide ketosynthase/chain length factor== | ==The ishigamide ketosynthase/chain length factor== | ||
<StructureSection load='6kxf' size='340' side='right'caption='[[6kxf]]' scene=''> | <StructureSection load='6kxf' size='340' side='right'caption='[[6kxf]], [[Resolution|resolution]] 1.98Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXF FirstGlance]. <br> | <table><tr><td colspan='2'>[[6kxf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXF FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxf OCA], [http://pdbe.org/6kxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxf RCSB], [http://www.ebi.ac.uk/pdbsum/6kxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxf ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYF:[(3~{R})-2,2-dimethyl-4-[[3-[2-[[(~{E})-oct-2-enoyl]amino]ethylamino]-3-oxidanylidene-propyl]amino]-3-oxidanyl-4-oxidanylidene-butyl]+dihydrogen+phosphate'>DYF</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxf OCA], [http://pdbe.org/6kxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxf RCSB], [http://www.ebi.ac.uk/pdbsum/6kxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxf ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In type II polyketide synthases (PKSs), the ketosynthase-chain length factor (KS-CLF) complex catalyzes polyketide chain elongation with the acyl carrier protein (ACP). Highly reducing type II PKSs, represented by IgaPKS, produce polyene structures instead of the well-known aromatic skeletons. Here, we report the crystal structures of the Iga11-Iga12 (KS-CLF) heterodimer and the covalently cross-linked Iga10=Iga11-Iga12 (ACP=KS-CLF) tripartite complex. The latter structure revealed the molecular basis of the interaction between Iga10 and Iga11-Iga12, which differs from that between the ACP and KS of Escherichia coli fatty acid synthase. Furthermore, the reaction pocket structure and site-directed mutagenesis revealed that the negative charge of Asp 113 of Iga11 prevents further condensation using a beta-ketoacyl product as a substrate, which distinguishes IgaPKS from typical type II PKSs. This work will facilitate the future rational design of PKSs. | |||
Structural basis for selectivity in a highly reducing type II polyketide synthase.,Du D, Katsuyama Y, Horiuchi M, Fushinobu S, Chen A, Davis TD, Burkart MD, Ohnishi Y Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0530-0. doi:, 10.1038/s41589-020-0530-0. PMID:32367018<ref>PMID:32367018</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6kxf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Burkart M]] | [[Category: Burkart, M]] | ||
[[Category: Chen A]] | [[Category: Chen, A]] | ||
[[Category: Davis T]] | [[Category: Davis, T]] | ||
[[Category: Du D]] | [[Category: Du, D]] | ||
[[Category: Fushinobu S]] | [[Category: Fushinobu, S]] | ||
[[Category: Horiuchi M]] | [[Category: Horiuchi, M]] | ||
[[Category: Katsuyama Y]] | [[Category: Katsuyama, Y]] | ||
[[Category: Ohnishi Y]] | [[Category: Ohnishi, Y]] | ||
[[Category: Polyketide synthase]] | |||
[[Category: Transferase]] | |||
Revision as of 06:00, 20 May 2020
The ishigamide ketosynthase/chain length factor
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