1b9z: Difference between revisions

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[[Image:1b9z.gif|left|200px]]
{{Seed}}
[[Image:1b9z.png|left|200px]]


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{{STRUCTURE_1b9z|  PDB=1b9z  |  SCENE=  }}  
{{STRUCTURE_1b9z|  PDB=1b9z  |  SCENE=  }}  


'''BACILLUS CEREUS BETA-AMYLASE COMPLEXED WITH MALTOSE'''
===BACILLUS CEREUS BETA-AMYLASE COMPLEXED WITH MALTOSE===




==Overview==
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The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b = 92.87 A, c = 65.93 A, and beta =101.95 degrees. The structures of free and maltose-bound beta-amylases were determined by X-ray crystallography at 2.1 and 2.5 A with R-factors of 0.170 and 0.164, respectively. The final model of the maltose-bound form comprises 516 amino acid residues, four maltose molecules, 275 water molecules, one Ca2+, one acetate, and one sulfate ion. The enzyme consists of a core (beta/alpha)8-barrel domain (residues 5-434) and a C-terminal starch-binding domain (residues 435-613). Besides the active site in the core where two maltose molecules are bound in tandem, two novel maltose-binding sites were found in the core L4 region and in the C-terminal domain. The structure of the core domain is similar to that of soybean beta-amylase except for the L4 maltose-binding site, whereas the C-terminal domain has the same secondary structure as domain E of cyclodextrin glucosyltransferase. These two maltose-binding sites are 32-36 A apart from the active site. These results indicate that the ability of B. cereus beta-amylase to digest raw starch can be attributed to the additional two maltose-binding sites.
The line below this paragraph, {{ABSTRACT_PUBMED_10353816}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_10353816}}


==About this Structure==
==About this Structure==
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[[Category: Shinke, R.]]
[[Category: Shinke, R.]]
[[Category: Utsumi, S.]]
[[Category: Utsumi, S.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:15:39 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:39:58 2008''

Revision as of 15:40, 30 June 2008

File:1b9z.png

Template:STRUCTURE 1b9z

BACILLUS CEREUS BETA-AMYLASE COMPLEXED WITH MALTOSE

Template:ABSTRACT PUBMED 10353816

About this Structure

1B9Z is a Single protein structure of sequence from Bacillus cereus. Full crystallographic information is available from OCA.

Reference

Structure of raw starch-digesting Bacillus cereus beta-amylase complexed with maltose., Mikami B, Adachi M, Kage T, Sarikaya E, Nanmori T, Shinke R, Utsumi S, Biochemistry. 1999 Jun 1;38(22):7050-61. PMID:10353816

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